NeuroMabSeq: high volume acquisition, processing, and curation of hybridoma sequences and their use in generating recombinant monoclonal antibodies and scFvs for neuroscience research.

Autor:	Mitchell KG; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA.; Bioinformatics Core, Genome Center, University of California Davis, CA., Gong B; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA., Hunter SS; Bioinformatics Core, Genome Center, University of California Davis, CA., Burkart-Waco D; DNA Technology Core, Genome Center, University of California Davis, CA., Gavira-O'Neill CE; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA., Templeton KM; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA., Goethel ME; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA., Bzymek M; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA., MacNiven LM; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA., Murray KD; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA.; Department of Psychiatry and Behavioral Sciences, University of California Davis School of Medicine, Davis, CA., Settles ML; Bioinformatics Core, Genome Center, University of California Davis, CA., Froenicke L; DNA Technology Core, Genome Center, University of California Davis, CA., Trimmer JS; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Jun 30. Date of Electronic Publication: 2023 Jun 30.
DOI:	10.1101/2023.06.28.546392
Abstrakt:	The Neuroscience Monoclonal Antibody Sequencing Initiative (NeuroMabSeq) is a concerted effort to determine and make publicly available hybridoma-derived sequences of monoclonal antibodies (mAbs) valuable to neuroscience research. Over 30 years of research and development efforts including those at the UC Davis/NIH NeuroMab Facility have resulted in the generation of a large collection of mouse mAbs validated for neuroscience research. To enhance dissemination and increase the utility of this valuable resource, we applied a high-throughput DNA sequencing approach to determine immunoglobulin heavy and light chain variable domain sequences from source hybridoma cells. The resultant set of sequences was made publicly available as searchable DNA sequence database ( neuromabseq.ucdavis.edu ) for sharing, analysis and use in downstream applications. We enhanced the utility, transparency, and reproducibility of the existing mAb collection by using these sequences to develop recombinant mAbs. This enabled their subsequent engineering into alternate forms with distinct utility, including alternate modes of detection in multiplexed labeling, and as miniaturized single chain variable fragments or scFvs. The NeuroMabSeq website and database and the corresponding recombinant antibody collection together serve as a public DNA sequence repository of mouse mAb heavy and light chain variable domain sequences and as an open resource for enhancing dissemination and utility of this valuable collection of validated mAbs.
Databáze:	MEDLINE
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