Rapid assessment of changes in phage bioactivity using dynamic light scattering.

Autor:	Dharmaraj T; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.; Sarafan ChEM-H, Stanford University, Stanford, CA 94305, USA., Kratochvil MJ; Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305., Pourtois JD; Hopkins Marine Station, Department of Biology, Stanford University, Pacific Grove, CA 93950, USA., Chen Q; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Hajfathalian M; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Hargil A; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Lin YH; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA., Evans Z; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Oromí-Bosch A; Felix Biotechnology, South San Francisco, CA, 94080., Berry JD; Felix Biotechnology, South San Francisco, CA, 94080., McBride R; Felix Biotechnology, South San Francisco, CA, 94080., Haddock NL; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Holman DR; Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., van Belleghem JD; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Chang TH; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Barr JJ; School of Biological Sciences, Monash University, Clayton, 3800, VIC, Australia., Lavigne R; Department of Biosystems, KU Leuven, Leuven, 3001, Belgium., Heilshorn SC; Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305., Blankenberg FG; Division of Pediatric Radiology and Nuclear Medicine, Department of Radiology, Lucile Packard Children's Hospital, Stanford, CA 94305, USA., Bollyky PL; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Jul 02. Date of Electronic Publication: 2023 Jul 02.
DOI:	10.1101/2023.07.02.547396
Abstrakt:	Extensive efforts are underway to develop bacteriophages as therapies against antibiotic-resistant bacteria. However, these efforts are confounded by the instability of phage preparations and a lack of suitable tools to assess active phage concentrations over time. Here, we use Dynamic Light Scattering (DLS) to measure changes in phage physical state in response to environmental factors and time, finding that phages tend to decay and form aggregates and that the degree of aggregation can be used to predict phage bioactivity. We then use DLS to optimize phage storage conditions for phages from human clinical trials, predict bioactivity in 50-year-old archival stocks, and evaluate phage samples for use in a phage therapy/wound infection model. We also provide a web-application (Phage-ELF) to facilitate DLS studies of phages. We conclude that DLS provides a rapid, convenient, and non-destructive tool for quality control of phage preparations in academic and commercial settings. Competing Interests: Competing Interest Statement: AO-B, JDB, RM are members of Felix Biotechnology, Inc., a phage therapy company. All other authors declare they have no competing interests.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu