A "Kidney-on-the-Chip" Model Composed of Primary Human Tubular, Endothelial, and White Blood Cells.

Autor:	Marschner JA; Renal Division, Department of Medicine IV, Hospital of the Ludwig Maximilians University, Munich, Germany.; Department of Pharmacy, Ludwig Maximilians University, Munich, Germany., Martin L; Renal Division, Department of Medicine IV, Hospital of the Ludwig Maximilians University, Munich, Germany., Wilken G; Renal Division, Department of Medicine IV, Hospital of the Ludwig Maximilians University, Munich, Germany., Melica ME; Department of Experimental and Clinical Biomedical Sciences 'Mario Serio', University of Florence, Florence, Italy., Anders HJ; Renal Division, Department of Medicine IV, Hospital of the Ludwig Maximilians University, Munich, Germany. Hans-Joachim.Anders@med.uni-muenchen.de.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2664, pp. 107-121.
DOI:	10.1007/978-1-0716-3179-9_8
Abstrakt:	State-of-the-art cell culture systems may enlist a variety of features to push the significance of in vitro models beyond classical 2D single cell culture; among them are the 3D scaffolds of organic or artificial materials, multi-cell setups, and the use of primary cells as source materials. Obviously, operational complexity increases with each additional feature and feasibility, whereas reproducibility may suffer.We report a multicellular setup using primary human cells and the Mimetas scaffold that aims to increase pathophysiological significance of in vitro culture and simultaneously allows for relatively high-throughput and easy handling. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu