Evaluation of dietary single probiotic isolates and probiotic multistrain consortia in growth performance, gut histology, gut microbiota, immune regulation, and infection resistance of Nile tilapia, Oreochromis niloticus, shows superior monostrain performance.

Autor: Melo-Bolívar JF; Universidad de La Sabana, Doctorado en Biociencias, Campus del Puente del Común, Km. 7, Autopista Norte de Bogotá, Chía, Cundinamarca, Colombia., Ruiz Pardo RY; Universidad de La Sabana, Doctorado en Biociencias, Campus del Puente del Común, Km. 7, Autopista Norte de Bogotá, Chía, Cundinamarca, Colombia., Quintanilla-Carvajal MX; Universidad de La Sabana, Doctorado en Biociencias, Campus del Puente del Común, Km. 7, Autopista Norte de Bogotá, Chía, Cundinamarca, Colombia., Díaz LE; Universidad de La Sabana, Doctorado en Biociencias, Campus del Puente del Común, Km. 7, Autopista Norte de Bogotá, Chía, Cundinamarca, Colombia., Alzate JF; Centro Nacional de Secuenciación Genómica- CNSG, Sede de Investigación Universitaria SIU, Grupo Pediaciencias, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia., Junca H; Microbiomas Foundation, Div. Ecogenomics & Holobionts, RG Microbial Ecology: Metabolism, Genomics & Evolution, Chía, Colombia., Rodríguez Orjuela JA; Universidad de La Sabana, Doctorado en Biociencias, Campus del Puente del Común, Km. 7, Autopista Norte de Bogotá, Chía, Cundinamarca, Colombia., Villamil Diaz LM; Universidad de La Sabana, Doctorado en Biociencias, Campus del Puente del Común, Km. 7, Autopista Norte de Bogotá, Chía, Cundinamarca, Colombia. Electronic address: luisa.villamil@unisabana.edu.co.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2023 Sep; Vol. 140, pp. 108928. Date of Electronic Publication: 2023 Jul 07.
DOI: 10.1016/j.fsi.2023.108928
Abstrakt: The probiotic potential of a designed bacterial consortia isolated from a competitive exclusion culture originally obtained from the intestinal contents of tilapia juveniles were evaluated on Nile tilapia alevins. The growth performance, intestinal histology, microbiota effects, resistance to Streptococcus agalactiae challenge, and immune response were assessed. In addition, the following treatments were included in a commercial feed: A12+M4+M10 (Lactococcus lactis A12, Priestia megaterium M4, and Priestia sp. M10), M4+M10 (P. megaterium M4, and Priestia sp. M10) and the single bacteria as controls; A12 (L. lactis A12), M4 (P. megaterium M4), M10 (Priestia sp. M10), also a commercial feed without any probiotic addition was included as a control. The results showed that all probiotic treatments improved the growth performance, intestinal histology, and resistance during experimental infection with S. agalactiae in comparison to the control fish. Also, the administration of probiotics resulted in the modulation of genes associated with the innate and adaptive immune systems that were non-dependent on microbial colonization. Surprisingly, L. lactis A12 alone induced benefits in fish compared to the microbial consortia, showing the highest increase in growth rate, survival during experimental infection with S. agalactiae, increased intestinal fold length, and the number of differentially expressed genes. Lastly, we conclude that a competitive exclusion culture is a reliable source of probiotics, and monostrain L. lactis A12 has comparable or even greater probiotic potential than the bacterial consortia.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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