In-situ synthesized V 2 CT x MXene-based immune tag for the electrochemical detection of Interleukin 6 (IL-6) from breast cancer cells.

Autor: Vessella T; Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA, 01609, USA., Zhang H; Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA, 01609, USA., Zhou Z; Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA, 01609, USA., Cui F; Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA, 01609, USA. Electronic address: feiyun@hrbmu.edu.cn., Zhou HS; Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA, 01609, USA. Electronic address: szhou@wpi.edu.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2023 Oct 01; Vol. 237, pp. 115512. Date of Electronic Publication: 2023 Jul 04.
DOI: 10.1016/j.bios.2023.115512
Abstrakt: Interleukin-6 (IL-6) is a proinflammatory cytokine with a critical role in immune regulation and treatment of many diseases, including breast cancer. Herein, we developed a novel V 2 CT x MXene-based immunosensor for rapid and accurate IL-6 detection. The chosen substrate was V 2 CT x , a 2-dimensional (2D) MXene nanomaterial with excellent electronic properties. Prussian blue (Fe 4 [Fe(CN) 6 ] 3 ), used for its electrochemical properties, and spindle-shaped gold nanoparticles (Au SSNPs), used to combine with antibodies, were in-situ synthesized on the surface of the MXene. The in-situ synthesis ensures a firm chemical connection compared to other tags formed by a less stable physical absorption. Inspired by a sandwich ELISA test, the modified V 2 CT x tag was captured by the electrode surface with cysteamine to detect the analyte, IL-6, after being attached with a capture antibody (cAb). Benefiting from an increased surface area, an enhanced charge transfer rate, and a firm connection of the tag, this biosensor exhibited excellent analytical performance. The high sensitivity, high selectivity, and wide detection range covering the IL-6 level of both healthy individuals and breast cancer patients were obtained to meet clinical demands. Herein, this V 2 CT x MXene-based immunosensor is a potential therapeutic and diagnostic point-of-care alternative to routine ELISA IL-6 detection methods.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: