Development of an antibacterial and antioxidative nanofibrous membrane using curcumin-loaded halloysite nanotubes for smart wound healing: In vitro and in vivo studies.

Autor: Shakiba M; Department of Chemistry, Amirkabir University of Technology, Tehran, Iran., Sheikhi M; Polymer Chemistry Research Laboratory, Department of Chemistry, University of Isfahan, Isfahan, Iran., Pahnavar Z; Department of Chemistry, University of Mazandaran, Babolsar, Iran., Tajiki A; Department of Chemistry, Amirkabir University of Technology, Tehran, Iran., Bigham A; Institute of Polymers, Composites and Biomaterials, National Research Council of Italy (IPCB-CNR), Viale John Fitzgerald Kennedy 54, Mostra d'Oltremare Padiglione 20, 80125 Naples, Italy; Department of Chemical, Materials and Production Engineering, University of Naples Federico II, Piazzale V. Tecchio 80, 80125 Naples, Italy. Electronic address: ashkan.bigham@ipcb.cnr.it., Foroozandeh A; Department of Chemistry, Amirkabir University of Technology, Tehran, Iran., Darvishan S; Department of Chemistry, Amirkabir University of Technology, Tehran, Iran., Pourmadadi M; School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran., Emadi H; Department of Chemistry, University of Mazandaran, Babolsar, Iran., Rezatabar J; Department of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran., Abdouss H; School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran., Abdouss M; Department of Chemistry, Amirkabir University of Technology, Tehran, Iran. Electronic address: phdabdouss44@aut.ac.ir.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2023 Jul 25; Vol. 642, pp. 123207. Date of Electronic Publication: 2023 Jul 05.
DOI: 10.1016/j.ijpharm.2023.123207
Abstrakt: Endowing wound dressings with drug delivery capability is a suitable strategy to transfer medicinal compounds locally to damaged skin layers. These dressings are especially useful for accelerating the healing rate in the cases of long-term treatment, and adding more functionalities to the platform. In this study, a wound dressing composed of polyamide 6, hyaluronic acid, and curcumin-loaded halloysite nanotubes (PA6/HA/HNT@Cur) was designed and fabricated for wound healing applications. The physicochemical properties of this platform were investigated through Fourier-transform infrared spectroscopy and field-emission scanning electron microscopy. Moreover, wettability, tensile strength, swelling, and in vitro degradation were assessed. The HNT@Cur was incorporated in the fibers in three concentrations and 1 wt% was found as the optimum concentration yielding desirable structural and mechanical properties. The loading efficiency of Cur on HNT was calculated to be 43 ± 1.8%, and the release profiles and kinetics of nanocomposite were investigated at physiological and acidic pH. In vitro antibacterial and antioxidation studies showed that the PA6/HA/HNT@Cur mat had strong antibacterial and antioxidation activities against gram-positive and -negative pathogens and reactive oxygen species, respectively. Desirable cell compatibility of the mat was found through MTT assay against L292 cells up to 72 h. Finally, the efficacy of the designed wound dressing was evaluated in vivo; after 14 days, the results indicated that the wound size treated with the nanocomposite mat significantly decreased compared to the control sample. This study proposed a swift and straightforward method for developing materials that might be utilized as wound dressings in clinical settings.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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