Proteomic research on new urinary biomarkers of renal disease in canine leishmaniosis: Survival and monitoring response to treatment.

Autor: González MA; Animal Medicine Department, University of Extremadura, 10003 Cáceres, Spain. Electronic address: marioalbgs@unex.es., Barrera-Chacón R; Animal Medicine Department, University of Extremadura, 10003 Cáceres, Spain., Peña FJ; Animal Medicine Department, University of Extremadura, 10003 Cáceres, Spain; Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, 10003 Cáceres, Spain., Belinchón-Lorenzo S; LeishmanCeres Laboratory (GLP Compliance Certified), Parasitology Unit, Veterinary Teaching Hospital, University of Extremadura, 10003 Cáceres, Spain., Robles NR; Nephrology Service, Badajoz University Hospital, University of Extremadura, 06080 Badajoz, Spain., Pérez-Merino EM; Animal Medicine Department, University of Extremadura, 10003 Cáceres, Spain., Martín-Cano FE; Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, 10003 Cáceres, Spain., Duque FJ; Animal Medicine Department, University of Extremadura, 10003 Cáceres, Spain.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2023 Aug; Vol. 161, pp. 180-190. Date of Electronic Publication: 2023 Jun 22.
DOI: 10.1016/j.rvsc.2023.06.016
Abstrakt: The objective of our study was to search for survival biomarkers (SB) and treatment response monitoring biomarkers (TRMB) in the urinary proteome of dogs with renal disease secondary to canine leishmaniosis (CanL), using UHPLC-MS/MS. The proteomic data are available via ProteomeXchange with identifier PXD042578. Initially, a group of 12 dogs was evaluated and divided into survivors (SG; n = 6) and nonsurvivors (NSG; n = 6). A total of 972 proteins were obtained from the evaluated samples. Then, bioinformatic analysis reduced them to 6 proteins like potential SB increased in the NSG, specifically, Haemoglobin subunit Alpha 1, Complement Factor I, Complement C5, Fibrinogen beta chain (fragment), Peptidase S1 domain-containing protein, and Fibrinogen gamma chain. Afterwards, SG was used to search for TRMB, studying their urine at 0, 30, and 90 days, and 9 proteins that decreased after treatment were obtained: Apolipoprotein E, Cathepsin B, Cystatin B, Cystatin-C-like, Lysozyme, Monocyte differentiation CD14, Pancreatitis-associated precursor protein, Profilin, and Protein FAM3C. Finally, enrichment analysis provided information about the biological mechanisms in which these proteins are involved. In conclusion, this study provides 15 new candidate urinary biomarkers and an improved understanding of the pathogenesis of kidney disease in CanL.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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