Bacterial cytological profiling reveals interactions between jumbo phage φKZ infection and cell wall active antibiotics in Pseudomonas aeruginosa.

Autor: Tsunemoto H; Division of Biological Sciences, University of California, San Diego, San Diego, CA, United States of America., Sugie J; Division of Biological Sciences, University of California, San Diego, San Diego, CA, United States of America., Enustun E; Division of Biological Sciences, University of California, San Diego, San Diego, CA, United States of America., Pogliano K; Division of Biological Sciences, University of California, San Diego, San Diego, CA, United States of America., Pogliano J; Division of Biological Sciences, University of California, San Diego, San Diego, CA, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Jul 07; Vol. 18 (7), pp. e0280070. Date of Electronic Publication: 2023 Jul 07 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0280070
Abstrakt: The emergence of antibiotic resistance in bacteria has led to the investigation of alternative treatments, such as phage therapy. In this study, we examined the interactions between the nucleus-forming jumbo phage ФKZ and antibiotic treatment against Pseudomonas aeruginosa. Using the fluorescence microscopy technique of bacterial cytological profiling, we identified mechanism-of-action-specific interactions between antibiotics that target different biosynthetic pathways and ФKZ infection. We found that certain classes of antibiotics strongly inhibited phage replication, while others had no effect or only mildly affected progression through the lytic cycle. Antibiotics that caused an increase in host cell length, such as the cell wall active antibiotic ceftazidime, prevented proper centering of the ФKZ nucleus via the PhuZ spindle at midcell, leading us to hypothesize that the kinetic parameters of the PhuZ spindle evolved to match the average length of the host cell. To test this, we developed a computational model explaining how the dynamic properties of the PhuZ spindle contribute to phage nucleus centering and why some antibiotics affect nucleus positioning while others do not. These findings provide an understanding of the molecular mechanisms underlying the interactions between antibiotics and jumbo phage replication.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Tsunemoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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