Digital Examination of LYmph node CYtopathology Using the Sydney system (DELYCYUS): An international, multi-institutional study.

Autor: Caputo A; Department of Pathology, University Hospital of Salerno, Salerno, Italy., Fraggetta F; Department of Pathology, Gravina and Santo Pietro Hospital, Caltagirone, Italy., Cretella P; Department of Advanced Biomedical Sciences, 'Federico II' University, Naples, Italy., Cozzolino I; Department of Mental and Physical Health and Preventive Medicine, Università Degli Studi Della Campania 'Luigi Vanvitelli', Naples, Italy., Eccher A; Department of Pathology and Diagnostics, University and Hospital Trust of Verona, Verona, Italy., Girolami I; Department of Pathology, Provincial Hospital of Bolzano, South Tyrolean Health Care Service-South Tyrol Health Authority, Bolzano-Bozen, Italy., Marletta S; Department of Diagnostics and Public Health, University and Hospital Trust of Verona, Verona, Italy., Troncone G; Department of Public Health, 'Federico II' University, Naples, Italy., Vigliar E; Department of Public Health, 'Federico II' University, Naples, Italy., Acanfora G; Department of Public Health, 'Federico II' University, Naples, Italy., Zarra KV; Pathology Department, Hospital Universitario Del Henares, Coslada, Spain., Torres Rivas HE; Pathology Department, Hospital Universitario Central de Asturias, Oviedo, Spain., Fadda G; Department of Human Pathology of the Adulthood and Developing Age 'Gaetano Barresi', Section of Pathology, University of Messina, Messina, Italy., Field A; Department of Anatomical Pathology, St Vincent's Hospital, University of New South Wales and University of Notre Dame, Sydney, New South Wales, Australia., Katz R; Department of Pathology, Tel HaShomer Hospital, Tel Aviv, Israel., Vielh P; Medipath and American Hospital of Paris, Paris, France., Eloy C; Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal., Rajwanshi A; All India Institute of Medical Sciences, Raebareli, India., Gupta N; Department of Cytopathology and Gynecologic Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India., Al-Abbadi M; Department of Pathology, Microbiology and Forensic Medicine, The University of Jordan, Amman, Jordan., Bustami N; Department of Pathology, Microbiology and Forensic Medicine, The University of Jordan, Amman, Jordan., Arar T; Department of Pathology, Microbiology and Forensic Medicine, The University of Jordan, Amman, Jordan., Calaminici M; Specialist Integrated Hematological Malignancy Diagnostic Service, Department of Cellular Pathology, Barts Health National Health Service Trust, England, UK.; Center for Hemato-Oncology, Barts Cancer Institute, London, UK., Raine JI; Specialist Integrated Hematological Malignancy Diagnostic Service, Department of Cellular Pathology, Barts Health National Health Service Trust, England, UK., Barroca H; Serviço de Anatomia Patológica, Hospital S João-Porto, Porto, Portugal., Canão PA; Serviço de Anatomia Patológica, Hospital S João-Porto, Porto, Portugal., Ehinger M; Department of Clinical Sciences, Pathology, Skane University Hospital, Lund University, Lund, Sweden., Rajabian N; Department of Clinical Sciences, Pathology, Skane University Hospital, Lund University, Lund, Sweden., Dey P; Department of Cytopathology and Gynecologic Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India., Medeiros LJ; Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA., El Hussein S; Department of Pathology, University of Rochester Medical Center, Rochester, New York, USA., Lin O; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA., D'Antonio A; Department of Pathology, Ospedale Del Mare, Napoli, Italy., Bode-Lesniewska B; Pathologie Institut Enge, Zurich, Switzerland., Rossi ED; Division of Anatomic Pathology and Histology, Catholic University Rome, Rome, Italy., Zeppa P; Department of Pathology, University Hospital of Salerno, Salerno, Italy.
Jazyk: angličtina
Zdroj: Cancer cytopathology [Cancer Cytopathol] 2023 Nov; Vol. 131 (11), pp. 679-692. Date of Electronic Publication: 2023 Jul 07.
DOI: 10.1002/cncy.22741
Abstrakt: Background: After a series of standardized reporting systems in cytopathology, the Sydney system was recently introduced to address the need for reproducibility and standardization in lymph node cytopathology. Since then, the risk of malignancy for the categories of the Sydney system has been explored by several studies, but no studies have yet examined the interobserver reproducibility of the Sydney system.
Methods: The authors assessed interobserver reproducibility of the Sydney system on 85 lymph node fine-needle aspiration cytology cases reviewed by 15 cytopathologists from 12 institutions in eight different countries, resulting in 1275 diagnoses. In total, 186 slides stained with Diff-Quik, Papanicolaou, and immunocytochemistry were scanned. A subset of the cases included clinical data and results from ultrasound examinations, flow cytometry immunophenotyping, and fluorescence in situ hybridization analysis. The study participants assessed the cases digitally using whole-slide images.
Results: Overall, the authors observed an almost perfect agreement of cytopathologists with the ground truth (median weighted Cohen κ = 0.887; interquartile range, κ = 0.210) and moderate overall interobserver concordance (Fleiss κ = 0.476). There was substantial agreement for the inadequate and malignant categories (κ = 0.794 and κ = 0.729, respectively), moderate agreement for the benign category (κ = 0.490), and very slight agreement for the suspicious (κ = 0.104) and atypical (κ = 0.075) categories.
Conclusions: The Sydney system for reporting lymph node cytopathology shows adequate interobserver concordance. Digital microscopy is an adequate means to assess lymph node cytopathology specimens.
(© 2023 The Authors. Cancer Cytopathology published by Wiley Periodicals LLC on behalf of American Cancer Society.)
Databáze: MEDLINE
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