The Opto-inflammasome in zebrafish as a tool to study cell and tissue responses to speck formation and cell death.
| Autor: | Hasel de Carvalho E; European Molecular Biology Laboratory, Heidelberg, Germany., Dharmadhikari SS; European Molecular Biology Laboratory, Heidelberg, Germany., Shkarina K; Department of Biochemistry, University of Lausanne, Lausanne, Switzerland., Xiong JR; Skin Research Laboratories, A*STAR, Singapore, Singapore., Reversade B; Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore.; Department of Pediatrics, National University of Singapore (NUS), Singapore, Singapore.; Medical Genetics Department, Koç University School of Medicine (KUSOM), Istanbul, Turkey.; Laboratory of Human Genetics & Therapeutics, Genome Institute of Singapore (GIS), A*STAR, Singapore, Singapore., Broz P; Department of Biochemistry, University of Lausanne, Lausanne, Switzerland., Leptin M; European Molecular Biology Laboratory, Heidelberg, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | ELife [Elife] 2023 Jul 07; Vol. 12. Date of Electronic Publication: 2023 Jul 07. |
| DOI: | 10.7554/eLife.86373 |
| Abstrakt: | The inflammasome is a conserved structure for the intracellular detection of danger or pathogen signals. As a large intracellular multiprotein signaling platform, it activates downstream effectors that initiate a rapid necrotic programmed cell death (PCD) termed pyroptosis and activation and secretion of pro-inflammatory cytokines to warn and activate surrounding cells. However, inflammasome activation is difficult to control experimentally on a single-cell level using canonical triggers. We constructed Opto-ASC, a light-responsive form of the inflammasome adaptor protein ASC (Apoptosis-Associated Speck-Like Protein Containing a CARD) which allows tight control of inflammasome formation in vivo. We introduced a cassette of this construct under the control of a heat shock element into zebrafish in which we can now induce ASC inflammasome (speck) formation in individual cells of the skin. We find that cell death resulting from ASC speck formation is morphologically distinct from apoptosis in periderm cells but not in basal cells. ASC-induced PCD can lead to apical or basal extrusion from the periderm. The apical extrusion in periderm cells depends on Caspb and triggers a strong Ca 2+ signaling response in nearby cells. Competing Interests: EH, SD, KS, JX, BR, PB, ML No competing interests declared (© 2023, Hasel de Carvalho et al.) |
| Databáze: | MEDLINE |
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