Development of microsatellite markers for the invasive mosquito Aedes koreicus.

Autor: Soresinetti L; Department of Biosciences and Pediatric Clinical Research Center 'Romeo Ed Enrica Invernizzi', University of Milan, 20133, Milan, Italy.; Department of Biology and Biotechnology, University of Pavia, 27100, Pavia, Italy., Arnoldi I; Department of Biosciences and Pediatric Clinical Research Center 'Romeo Ed Enrica Invernizzi', University of Milan, 20133, Milan, Italy.; Department of Biology and Biotechnology, University of Pavia, 27100, Pavia, Italy.; University School of Advanced Studies Pavia, IUSS, 27100, Pavia, Italy.; Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, 20133, Milan, Italy., Negri A; Department of Biosciences and Pediatric Clinical Research Center 'Romeo Ed Enrica Invernizzi', University of Milan, 20133, Milan, Italy.; Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, 20133, Milan, Italy.; Department of Environmental Biology, Sapienza University of Rome, Via Dei Sardi 70, 00185, Rome, Italy., Naro G; Department of Biosciences and Pediatric Clinical Research Center 'Romeo Ed Enrica Invernizzi', University of Milan, 20133, Milan, Italy., Michelutti A; Istituto Zooprofilattico Sperimentale Delle Venezie, 35020, Legnaro, Italy., Montarsi F; Istituto Zooprofilattico Sperimentale Delle Venezie, 35020, Legnaro, Italy., Mosca A; Istituto Per Le Piante da Legno E L 'Ambiente, I.P.L.A. S.P.A, 10132, Turin, Italy., Bandi C; Department of Biosciences and Pediatric Clinical Research Center 'Romeo Ed Enrica Invernizzi', University of Milan, 20133, Milan, Italy.; University School of Advanced Studies Pavia, IUSS, 27100, Pavia, Italy.; Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, 20133, Milan, Italy., Gabrieli P; Department of Biosciences and Pediatric Clinical Research Center 'Romeo Ed Enrica Invernizzi', University of Milan, 20133, Milan, Italy. paolo.gabrieli@unimi.it.; University School of Advanced Studies Pavia, IUSS, 27100, Pavia, Italy. paolo.gabrieli@unimi.it.; Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, 20133, Milan, Italy. paolo.gabrieli@unimi.it., Epis S; Department of Biosciences and Pediatric Clinical Research Center 'Romeo Ed Enrica Invernizzi', University of Milan, 20133, Milan, Italy. sara.epis@unimi.it.; University School of Advanced Studies Pavia, IUSS, 27100, Pavia, Italy. sara.epis@unimi.it.; Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, 20133, Milan, Italy. sara.epis@unimi.it.
Jazyk: angličtina
Zdroj: Parasites & vectors [Parasit Vectors] 2023 Jul 06; Vol. 16 (1), pp. 223. Date of Electronic Publication: 2023 Jul 06.
DOI: 10.1186/s13071-023-05823-z
Abstrakt: Background: Aedes koreicus is a mosquito species native to East Asia which has recently invaded several countries in Europe. In Italy, this mosquito was first detected in the North-East in 2011 and is now widely distributed in the entire northern part of the country. The development of specific genetic markers, such as microsatellites, is necessary to uncover the dispersal routes of this mosquito from its native areas and, eventually, to plan future control interventions.
Methods: Available raw sequences of genomic DNA of Ae. koreicus were screened in silico using BLASTn to identify possible microsatellite-containing sequences. Specific primer pairs were then designed, and their efficiency was determined through polymerase chain reaction (PCR) on 32 individuals of Ae. koreicus collected in Italy. PCR conditions were optimised in three multiplex reactions. Genotyping of individual mosquitoes was performed on both single and multiplex PCR reactions. Finally, analysis of intra-population variation was performed to assess the level of polymorphism of the markers.
Results: Mosquito genotyping provided consistent results in both single and multiplex reactions. Out of the 31 microsatellite markers identified in the Ae. koreicus genome raw sequences, 11 were polymorphic in the examined mosquito samples.
Conclusions: The results show that the 11 microsatellite markers developed here hold potential for investigating the genetic structure of Ae. koreicus populations. These markers could thus represent a novel and useful tool to infer the routes of invasion of this mosquito species into Europe and other non-native areas.
(© 2023. The Author(s).)
Databáze: MEDLINE
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