Three-dimensional cultures of gingival fibroblasts on fibrin-based scaffolds for gingival augmentation: A proof-of-concept study.

Autor: Asad MM; Department of Preventive Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan., Abdelhafez RS; Department of Preventive Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: rsabdelhafez@just.edu.jo., Barham R; Cell Therapy Center, the University of Jordan, Amman, Jordan., Abdaljaleel M; Department of Pathology, Microbiology, and Forensic Medicine, Faculty of Medicine, the University of Jordan and Jordan University Hospital, Amman, Jordan., Alkurdi B; Cell Therapy Center, the University of Jordan, Amman, Jordan., Al-Hadidi S; Cell Therapy Center, the University of Jordan, Amman, Jordan., Zalloum S; Cell Therapy Center, the University of Jordan, Amman, Jordan., Ismail MM; Cell Therapy Center, the University of Jordan, Amman, Jordan., Buqain R; Cell Therapy Center, the University of Jordan, Amman, Jordan., Jafar H; Department of Anatomy and Histology, School of Medicine, The University of Jordan, Amman, Jordan., Ababneh NA; Cell Therapy Center, the University of Jordan, Amman, Jordan. Electronic address: nidaaanwar@gmail.com.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2023 Oct; Vol. 154, pp. 105754. Date of Electronic Publication: 2023 Jun 20.
DOI: 10.1016/j.archoralbio.2023.105754
Abstrakt: Objective: Gingival tissue regeneration is associated with several challenges. Tissue engineering regenerates the different components of the tissues, providing three major elements: living cells, appropriate scaffolds, and tissue-inducing substances. This study aimed to regenerate the gingival connective tissue in vitro, using human gingival fibroblasts cultured in three-dimensional fibrin gel scaffolds.
Design: Human gingival fibroblasts were seeded in a novel three-dimensional fibrin gel scaffold and maintained in two media types: platelet lysate media (control) and collagen-stimulating media (test). Cellular viability and proliferation were assessed, and the production of collagen and other extracellular matrix components in these constructs was investigated and compared.
Results: Human gingival fibroblasts cultured in three-dimensional cultures were metabolically active and proliferated in both media. Furthermore, histologic sections, scanning electron microscopy, and quantitative polymerase chain reaction confirmed the production of higher levels of collagen and other extracellular matrix fibers in three-dimensional constructs cultured in collagen-stimulating media.
Conclusions: Culturing human gingival fibroblasts in a novel three-dimensional fibrin gel scaffold containing collagen-stimulating media resulted in a tissue-equivalent construct that mimics human gingival connective tissue. The impact of these results should be considered for further investigations, which may help to develop a compatible scaffold for gingival soft tissue regeneration and treatment of mucogingival deformities.
Competing Interests: Declaration of Competing Interest This study was supported by a grant from Jordan university of Science and technology, Irbid, Jordan. All authors report no conflicts of interest related to this study. No one of the authors had received any financial support for the research or had any commercial relationship with any of the mentioned companies. All the materials that have been used in this research were bought by the fund of the university.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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