Molecular characterization suggests kinetic modulation of expression of accessory viral protein, W, in Newcastle disease virus infected DF1 cells.

Autor: Nayak BN; National Institute of Animal Biotechnology, Hyderabad, Telangana India.; Regional Centre for Biotechnology, New Delhi, India., Rajagopal K; National Institute of Animal Biotechnology, Hyderabad, Telangana India., Shunmugasundaram R; National Institute of Animal Biotechnology, Hyderabad, Telangana India., Rao PL; National Institute of Animal Biotechnology, Hyderabad, Telangana India., Vaidyanathan S; National Institute of Animal Biotechnology, Hyderabad, Telangana India., Subbiah M; National Institute of Animal Biotechnology, Hyderabad, Telangana India.; Regional Centre for Biotechnology, New Delhi, India.
Jazyk: angličtina
Zdroj: Virusdisease [Virusdisease] 2023 Jun; Vol. 34 (2), pp. 236-247. Date of Electronic Publication: 2023 May 02.
DOI: 10.1007/s13337-023-00813-2
Abstrakt: Viruses adopt strategies to efficiently utilize their compact genome. Members of the family Paramyxoviridae , exhibit a cotranscriptional RNA editing mechanism wherein polymerase stuttering generates accessory proteins from Phosphoprotein ( P ) gene. Newcastle disease virus (NDV), an avian paramyxovirus, expresses two accessory proteins, V and W, by RNA editing. While P and V proteins are well studied, very little is known about W protein. Recent studies confirmed W protein expression in NDV and the unique subcellular localization of W proteins of virulent and avirulent NDV. We characterized the W protein of NDV strain Komarov, a moderately virulent vaccine strain. W mRNA expression ranged between 7 and 9% of total P gene transcripts similar to virulent NDV. However, W protein expression, detectable by 6 h, peaked at 24 h and dropped by 48 h post infection in DF1 cells indicating a kinetically regulated expression by the virus. The W protein localized in the nucleus and by mutations, a strong nuclear localization signal was identified in the C-terminal region of W protein. The viral growth kinetics study suggested neither supplementation of W protein nor subcellular localization pattern of the supplemented W protein influenced viral replication in vitro similar to that noticed in avirulent NDV. A cytoplasmic mutant of W protein localized in cytoplasm unlike specific mitochondrial colocalization as recorded in velogenic NDV strain SG10 indicating a possible role of W protein in determining the viral pathogenicity. This study describes for the first time, the distinct features of W protein of moderately virulent NDV.
Supplementary Information: The online version contains supplementary material available at 10.1007/s13337-023-00813-2.
Competing Interests: Conflict of interestThe authors declare that they have no competing interests.
(© The Author(s), under exclusive licence to Indian Virological Society 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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