Lipopolysaccharide-induced sickness behavior is not altered in male Fmr1-deficient mice.

Autor: Santana-Coelho D; Department of Psychology and Neuroscience, Baylor University, Waco, Texas, USA., Hodges SL; Institute of Biomedical Studies, Baylor University, Waco, Texas, USA.; Department of Biology, Baylor University, Waco, Texas, USA., Quintero SI; Department of Psychology and Neuroscience, Baylor University, Waco, Texas, USA., Womble PD; Department of Psychology and Neuroscience, Baylor University, Waco, Texas, USA., Sullens DG; Department of Psychology and Neuroscience, Baylor University, Waco, Texas, USA., Narvaiz DA; Department of Psychology and Neuroscience, Baylor University, Waco, Texas, USA., Herrera R; Department of Psychology and Neuroscience, Baylor University, Waco, Texas, USA., Sekeres MJ; Department of Psychology and Neuroscience, Baylor University, Waco, Texas, USA., Lugo JN; Department of Psychology and Neuroscience, Baylor University, Waco, Texas, USA.; Institute of Biomedical Studies, Baylor University, Waco, Texas, USA.; Department of Biology, Baylor University, Waco, Texas, USA.
Jazyk: angličtina
Zdroj: Brain and behavior [Brain Behav] 2023 Aug; Vol. 13 (8), pp. e3142. Date of Electronic Publication: 2023 Jul 05.
DOI: 10.1002/brb3.3142
Abstrakt: Objectives: Fragile X syndrome is the main monogenetic cause of intellectual disability and autism. Alterations in the immune system are commonly found in these developmental disorders. We and others have demonstrated that Fmr1 mutant mice present an altered response to immune stimuli. However, whether this altered immune response can influence the Fmr1 mutant behavioral outcomes in response to inflammation has not been fully investigated.
Materials and Methods: In the current study, we examine the behavioral sickness response of male wildtype and knockout  mice to the innate immune stimulus lipopolysaccharide (LPS) (0.1 mg/kg) to determine if Fmr1 mutants have altered sickness behavior. We used an enzyme-linked immunosorbent assay (ELISA) to measure changes in the cytokine interleukin-6 (IL-6) to determine that inflammation was induced in the mice. Sickness behavior was assessed in a wheel-running paradigm, and a tail suspension test was used to assess the depressive-like phenotype that follows sickness behavior in response to LPS.
Results: The ELISA using blood serum confirmed a significant increase in IL-6 in mice that were treated with LPS. Treated Fmr1 mutants exhibited decreased distance traveled in the wheel running after LPS administration, similar to treated controls. Another cohort of animals treated with LPS were tested in the tail suspension test and exhibited no alterations in immobility time in response to LPS.
Conclusion: Together, our data suggest that Fmr1 mutant mice do not have altered sickness behavior in response to a low dose of LPS.
(© 2023 The Authors. Brain and Behavior published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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