Synthesis and characterization of n-phosphonium chitosan and its virucidal activity evaluation against coronavirus.

Autor: de Moura Junior CF; School of Chemical Engineering, Department of Materials and Bioprocess Engineering, University of Campinas, Av. Albert Einstein, Campinas 13083-852, Brazil., Ochi D; School of Chemical Engineering, Department of Materials and Bioprocess Engineering, University of Campinas, Av. Albert Einstein, Campinas 13083-852, Brazil., Freitas ED; School of Chemical Engineering, Department of Materials and Bioprocess Engineering, University of Campinas, Av. Albert Einstein, Campinas 13083-852, Brazil., Kerwald J; School of Chemical Engineering, Department of Materials and Bioprocess Engineering, University of Campinas, Av. Albert Einstein, Campinas 13083-852, Brazil., d'Ávila MA; School of Mechanical Engineering, Department of Manufacturing and Materials Engineering, University of Campinas, Campinas 13083-860, Brazil., Beppu MM; School of Chemical Engineering, Department of Materials and Bioprocess Engineering, University of Campinas, Av. Albert Einstein, Campinas 13083-852, Brazil. Electronic address: beppu@unicamp.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Aug 15; Vol. 246, pp. 125665. Date of Electronic Publication: 2023 Jul 03.
DOI: 10.1016/j.ijbiomac.2023.125665
Abstrakt: Despite the worldwide vaccination effort against COVID-19, the demand for biocidal materials has increased. One promising solution is the chemical modification of polysaccharides, such as chitosan, which can provide antiviral activity through the insertion of cationic terminals. In this study, chitosan was modified with (4-carboxybutyl) triphenylphosphonium bromide to create N-phosphonium chitosan (NPCS), a quaternized derivative. The resulting NPCS samples with three degrees of substitution (15.6 %, 19.8 % and 24.2 %) were characterized and found to have improved solubility in water and alkaline solutions but reduced thermal stability. The particles zeta potential exhibits positive charges and is directly correlated with the degree of substitution of the derivative. In virucidal assays, all NPCS samples were able to inhibit 99.999 % of the MHV-3 coronavirus within 5 min at low concentrations of 0.1 mg/mL, while maintaining low cytotoxicity to L929 cells. In addition to its potential application against current coronavirus strains, NPCS could also be valuable in combating future pandemics caused by other viral pathogens. The antiviral properties of NPCS make it a promising material for use in coating surface and personal protective equipment to limit the spread of disease-causing viruses.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest. The authors are grateful for the financial support of the funding agencies CAPES and CNPq.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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