Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display.

Autor:	Arras P; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany., Zimmermann J; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany.; Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Darmstadt, Germany., Lipinski B; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany.; Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Darmstadt, Germany., Yanakieva D; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany., Klewinghaus D; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany., Krah S; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany., Kolmar H; Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Darmstadt, Germany., Pekar L; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany., Zielonka S; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany. Stefan.Zielonka@merckgroup.com.; Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Darmstadt, Germany. Stefan.Zielonka@merckgroup.com.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2681, pp. 113-129.
DOI:	10.1007/978-1-0716-3279-6_8
Abstrakt:	Cattle are known for their repertoire of antibodies harboring extremely long CDR3H regions that form extensive "knob on stalk" cysteine-rich structures. The compact knob domain allows for the recognition of epitopes potentially not accessible to classical antibodies. To effectively access the potential of bovine-derived antigen-specific ultra-long CDR3 antibodies, a straightforward and effective high-throughput method based on yeast surface display and fluorescence-activated cell sorting is described. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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