The effect of a preparation containing glycocholic acid on the biocidal efficacy of sodium hypochlorite in a biofilm laboratory model.

Autor: Haddad Y; Private Practice, Welland, Ontario, Canada., Chan WC; Private Practice, Sydney, New South Wales, Australia., Ha WN; Department of Endodontics, Sydney Dental School, University of Sydney, Sydney, New South Wales, Australia., Conde A; Postgraduate Program in Endodontics, European University of Madrid, Madrid, Spain., Estevez R; Postgraduate Program in Endodontics, European University of Madrid, Madrid, Spain., Lopez AP; Postgraduate Program in Endodontics, European University of Madrid, Madrid, Spain., Gates GC; Adelaide Dental School, University of Adelaide, Adelaide, South Australia, Australia., Rossi-Fedele G; Adelaide Dental School, University of Adelaide, Adelaide, South Australia, Australia.
Jazyk: angličtina
Zdroj: Australian endodontic journal : the journal of the Australian Society of Endodontology Inc [Aust Endod J] 2023 Dec; Vol. 49 (3), pp. 503-511. Date of Electronic Publication: 2023 Jul 05.
DOI: 10.1111/aej.12771
Abstrakt: This study assessed the antimicrobial effect of sodium hypochlorite (NaOCl) mixtures combined with Keratobacter (KB) using an engineered biofilm root canal model. Clinical and reagent grade NaOCl were mixed with KB (9:1-vol/vol) to assess pH values over 1 min to select the ideal solution with a pH just below the pKa of hypochlorous acid. The samples were randomly divided into five groups: 1% and 4% NaOCl reagents, a mixture of NaOCl:KB using 1% and 4% NaOCl reagents and distilled water. Outcome measures were colony-forming units (CFUs/mL) and positive/negative cultures. No significant differences were observed in the pairwise comparisons between 1%, 4% NaOCl and 4% NaOCl+KB for the outcome CFUs/mL. Only 4% NaOCl presented with negative cultures in all samples, whereas 1% NaOCl and 4% NaOCl+KB had similar results (54% vs. 40%). The addition of KB has a limited effect on the antimicrobial efficacy of 4% NaOCl in this laboratory model.
(© 2023 The Authors. Australian Endodontic Journal published by John Wiley & Sons Australia, Ltd on behalf of Australian Society of Endodontology Inc.)
Databáze: MEDLINE
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