Bringing pathology to nanomedicine: a comparison of in vivo toxicity of polymeric nanoparticle carriers with and without chitosan coating.

Autor: Poulios C; Department of Pathology, Faculty of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece. dr.poulios@hotmail.com.; European Society of Pathology, Brussels, Belgium. dr.poulios@hotmail.com., Karagkiozaki V; Laboratory of Thin Films, Nanobiomaterials-Nanosystems and Nanometrology, Faculty of Physics, Aristotle University of Thessaloniki, Thessaloniki, Greece.; BL NanoBiomed, Thessaloniki, Greece., Kapoukranidou D; Department of Physiology, Faculty of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece., Chakim Z; Laboratory of Thin Films, Nanobiomaterials-Nanosystems and Nanometrology, Faculty of Physics, Aristotle University of Thessaloniki, Thessaloniki, Greece.; BL NanoBiomed, Thessaloniki, Greece., Zarampoukas T; Department of Pathology, Faculty of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece., Foroglou N; Department of Neurosurgery, AHEPA University Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece., Logothetidis S; Laboratory of Thin Films, Nanobiomaterials-Nanosystems and Nanometrology, Faculty of Physics, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Jazyk: angličtina
Zdroj: Virchows Archiv : an international journal of pathology [Virchows Arch] 2023 Dec; Vol. 483 (6), pp. 775-786. Date of Electronic Publication: 2023 Jul 04.
DOI: 10.1007/s00428-023-03581-y
Abstrakt: Over the last years, there has been an increasing number of proposals for the use of nanomaterials in medicine. The safety of novel technologies must be verified, prior to their clinical application. Pathology has much to contribute towards this end. In this study, we compared the in vivo toxicity effects of poly- (lactic-co-glycolic acid) nanoparticles with and without chitosan shell. Both nanoparticle types were loaded with curcumin. The nanoparticles were assessed in vitro for potential cytotoxicity with cell viability studies. For the in vivo test, 36 adult Wistar rats were used, four of which were the control group. The remaining 32 were divided into 2 groups, each of which was administered differentially coated drug carriers: (A) nanoparticles without chitosan coating and (B) nanoparticles with chitosan coating. For both groups, the subcutaneous route was used for administration. Each group was further divided into 2 sub-groups of 8 animals each. The animals of the first sub-groups were sacrificed 24 h after the injection and those of the second on the 7th day. The control group was also divided into 2 subgroups of 2 animals each. At the appointed post-administrative date, the rats were sacrificed, and specimens from the brain, liver, kidneys, heart, stomach, lungs, and from the skin at the injection site were collected and studied histopathologically. The evaluation of both in vitro and in vivo testing shows that nanoparticles with chitosan have significantly less, if any, toxic effects compared to those without chitosan.
(© 2023. The Author(s).)
Databáze: MEDLINE
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