Downregulation of circ-Foxo3 in breast cancer stem-like cells.

Autor: Kamalabadi-Farahani M; Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran. kamalabadi@shmu.ac.ir., Atashi A; Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran., Eslami MM; Department of Hematology, School of Medicine, Tarbiat Modares University (TMU), Tehran, Iran.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2023 Jul 03; Vol. 16 (1), pp. 132. Date of Electronic Publication: 2023 Jul 03.
DOI: 10.1186/s13104-023-06405-z
Abstrakt: Objective: Cancer cells having stem cell characteristics are linked to metastasis and relapse in breast cancer. Circ-Foxo3, as a circular RNA, has been linked to the breast cancer lethal traits. This study's objective was to assess circ-Foxo3 expression in breast cancer stem-like cells. After isolation from tumor mass, breast cancer cells were subjected to the reliable in vitro assay of spheroid formation to determine the presence cancer stem cells (CSCs). We used a quantitative real-time polymerase chain reaction to examine circ-Foxo3 expression in spheroids.
Results: Circ-Foxo3 expression was significantly downregulated in spheroid-forming tumor cells, according to our data. This study demonstrated that breast CSCs have downregulated circ-Foxo3 expression, which may allow these cells to evade apoptosis. A precise analysis of this circRNA's role could be exploited to develop focused therapeutic approaches to fight breast CSCs.
(© 2023. The Author(s).)
Databáze: MEDLINE
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