Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics.

Autor: Bootz-Maoz H; The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.; Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel., Simon A; The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.; Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel., Del Mare-Roumani S; The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.; Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel., Bennet Y; The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.; Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel., Toister E; The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.; Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel., Romano H; The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.; Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel., Zheng D; Systems Immunology Department, Weizmann Institute of Science, 234 Herzl Street, Rehovot, 7610001, Israel., Amidror S; The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.; Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel., Elinav E; Systems Immunology Department, Weizmann Institute of Science, 234 Herzl Street, Rehovot, 7610001, Israel.; Microbiome & Cancer Division, Deutsches Krebsforschungszentrum (DKFZ), Neuenheimer Feld 280, 69120, Heidelberg, Germany., Yissachar N; The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel. nissan.yissachar@biu.ac.il.; Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel. nissan.yissachar@biu.ac.il.
Jazyk: angličtina
Zdroj: NPJ biofilms and microbiomes [NPJ Biofilms Microbiomes] 2023 Jul 03; Vol. 9 (1), pp. 44. Date of Electronic Publication: 2023 Jul 03.
DOI: 10.1038/s41522-023-00409-0
Abstrakt: The intestinal epithelial barrier facilitates homeostatic host-microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.
(© 2023. The Author(s).)
Databáze: MEDLINE
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