Part II: Impedance-based DNA biosensor for detection of isolated strains of phytopathogen Ralstonia solanacearum.

Autor: Patel R; Center for Research in Nanotechnology and Science, Indian Institute of Technology Bombay, Mumbai 400076, India. Electronic address: rhea.patel@iitb.ac.in., Vinchurkar M; Department of Electrical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, India., Shaikh AM; Department of Electrical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, India., Patkar R; Department of Electrical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, India., Adami A; Center for Sensors & Devices, Fondazione Bruno Kessler (FBK), Trento, Italy., Giacomozzi F; Center for Sensors & Devices, Fondazione Bruno Kessler (FBK), Trento, Italy., Ramesh R; Plant Pathology, ICAR- Central Coastal Agricultural Research Institute, Old Goa, Goa 403402, India., Pramanick B; School of Electrical Sciences and Centre of Excellence in Particulates Colloids and Interfaces, Indian Institute of Technology Goa, Goa 403401, India., Lorenzelli L; Center for Sensors & Devices, Fondazione Bruno Kessler (FBK), Trento, Italy., Baghini MS; Department of Electrical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2023 Oct; Vol. 153, pp. 108500. Date of Electronic Publication: 2023 Jun 30.
DOI: 10.1016/j.bioelechem.2023.108500
Abstrakt: In Part I, we demonstrated the complete development of a label-free, ultra-low sample volume requiring DNA-based biosensor to detect Ralstonia solanacearum, an aerobic non-spore-forming, Gram-negative, plant pathogenic bacterium, using non-faradaic electrochemical impedance spectroscopy (nf-EIS). We also presented the sensor's sensitivity, specificity, and electrochemical stability. In this article, we highlight the specificity study of the developed DNA-based impedimetric biosensor to detect various strains of R. solanacearum. We have collected seven isolates of R. solanacearum isolated from locally infected host plants (eggplant, potato, tomato, chilli, and ginger) from different parts of Goa, India. The pathogenicity of these isolates was tested on the eggplant, and the pathogen was confirmed by microbiological plating and polymerase chain reaction (PCR). We further report the insight into the DNA hybridization on the surface of Interdigitated Electrodes (IDEs) and the expansion of the Randles model for more accurate analysis. The interpretation of the sensor specificity is clearly demonstrated by the capacitance change observed at the electrode-electrolyte interface.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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