Multiplexed CRISPR-based Methods for Pathogen Nucleic Acid Detection.

Autor: Lamb CH; Department of Molecular Biology, Princeton University, Princeton, NJ, USA., Kang B; Department of Molecular Biology, Princeton University, Princeton, NJ, USA., Myhrvold C; Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
Jazyk: angličtina
Zdroj: Current opinion in biomedical engineering [Curr Opin Biomed Eng] 2023 Sep; Vol. 27. Date of Electronic Publication: 2023 May 20.
DOI: 10.1016/j.cobme.2023.100471
Abstrakt: Bacterial and viral pathogens are devastating to human health and well-being. In many regions, dozens of pathogen species and variants co-circulate. Thus, it is important to detect many different species and variants of pathogens in a given sample through multiplexed detection methods. CRISPR-based nucleic acid detection has shown to be a promising step towards an easy-to-use sensitive, specific, and high-throughput method to detect nucleic acids from DNA and RNA viruses and bacteria. Here, we review the current state of multiplexed nucleic acid detection methods with a focus on CRISPR-based methods. We also look toward the future of multiplexed point-of-care diagnostics.
Competing Interests: Declaration of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Competing Interests: C.M. is a co-founder of Carver Biosciences, a startup company developing Cas13-based antivirals, and holds equity in Carver Biosciences. The remaining authors declare no conflict of interest.
Databáze: MEDLINE
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