Seroprevalence, and molecular detection of Brucella abortus in cattle tissues from an abattoir in Namibia.

Autor: Madzingira O; Department of Paraclinical Sciences, School of Veterinary Medicine, Faculty of Health Sciences and Veterinary Medicine, University of Namibia, Windhoek, Namibia.; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., Aikukutu G; Central Veterinary Laboratory, Directorate of Veterinary Services, Ministry of Agriculture, Water and Land Reform, Windhoek, Namibia., Kandongo F; Directorate of Veterinary Services, Ministry of Agriculture, Water and Land Reform, Windhoek, Namibia., Kolo FB; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa., Khaiseb S; Central Veterinary Laboratory, Directorate of Veterinary Services, Ministry of Agriculture, Water and Land Reform, Windhoek, Namibia., Tjipura-Zaire G; Central Veterinary Laboratory, Directorate of Veterinary Services, Ministry of Agriculture, Water and Land Reform, Windhoek, Namibia., Kabajani JN; Central Veterinary Laboratory, Directorate of Veterinary Services, Ministry of Agriculture, Water and Land Reform, Windhoek, Namibia., Musilika-Shilongo A; Directorate of Veterinary Services, Ministry of Agriculture, Water and Land Reform, Windhoek, Namibia., van Heerden H; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Jazyk: angličtina
Zdroj: Infection ecology & epidemiology [Infect Ecol Epidemiol] 2023 Jun 28; Vol. 13 (1), pp. 2229589. Date of Electronic Publication: 2023 Jun 28 (Print Publication: 2023).
DOI: 10.1080/20008686.2023.2229589
Abstrakt: Brucellosis is a worldwide zoonosis that is endemic in Namibia. This study estimated seroprevalence of brucellosis, and determined the presence of Brucella infection in slaughtered cattle using the genus-specific 16-23S rRNA interspacer PCR (ITS-PCR), and the species-specific AMOS-PCR. Between December 2018 and May 2019, sera ( n = 304), pooled lymph nodes ( n = 304), and individual spleen ( n = 304) were collected from slaughtered cattle from 52 farms. Sera were tested for anti-Brucella antibodies using the Rose Bengal test (RBT), and the complement fixation test (CFT). Seroprevalence was 2.3% (7/304) (RBT) and 1.6% (5/304) (CFT). Prevalence of positive herds was 9.6% (5/52). Lymph node ( n = 200) and spleen ( n = 200) samples from seronegative cattle tested negative for Brucella spp. DNA on ITS-PCR, but Brucella spp. DNA was detected in lymph nodes (85.7%, 6/7) and spleen (85.7%, 6/7) from RBT positive cattle. ITS-PCR confirmed isolates from lymph node (51.4%, 4/7) and spleen (85.7%, 6/7) as Brucella spp.; while AMOS-PCR and Brucella abortus species specific (BaSS) PCR confirmed the isolates as Brucella abortus , and field strains, respectively. Provision of adequate protective gear, and the promotion of brucellosis awareness among abattoir workers is recommended to prevent zoonotic infection.
Competing Interests: No potential conflict of interest was reported by the authors.
(© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)
Databáze: MEDLINE
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