Hierarchical decision model for in vitro bilirubin content prediction from absorption spectrum of whole blood.
| Autor: | Mishra S; Indian Institute of Technology, Department of Biological Sciences and Bioengineering, Kanpur, Uttar Pradesh, India., Wanare H; Indian Institute of Technology, Centre of Lasers and Photonics, Kanpur, Uttar Pradesh, India.; Indian Institute of Technology, Department of Physics, Kanpur, Uttar Pradesh, India. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Journal of biomedical optics [J Biomed Opt] 2023 Jun; Vol. 28 (6), pp. 067001. Date of Electronic Publication: 2023 Jun 29. |
| DOI: | 10.1117/1.JBO.28.6.067001 |
| Abstrakt: | Significance: Bilirubin forms by the breakdown of heme proteins in the liver, but a newborn's sluggish liver can lead to elevated serum bilirubin levels that cross the blood-brain barrier and result in kernicterus. Earlier studies have used the 400 to 500 nm optical wavelength range to characterize the bilirubin content. There is not a universally established correlation among other wavelengths and the amount of bilirubin in clinical whole blood samples. Aim: We demonstrated that the amount of bilirubin could be quantified with ∼ 82 % accuracy in a label-free, self-referenced manner using only a few wavelengths, viz. 468, 492, 500, 560, 605, 645, 660, and 675 nm, wherein band-averaged absorption measurements are used. Approach: We addressed the above problem by conducting a preliminary study containing 50 neonates through an absorption spectrum measurement of whole blood in 3 to 5 μ l samples from the neonates. We constructed a hierarchical decision method that first grossly divides the 30 neonates of the training set into < 10 mg / dl and ≥ 10 mg / dl bilirubin level cohorts. A subsequent boundary condition further divides the ≥ 10 mg / dl group into two > 15 mg / dl and ≤ 15 mg / dl bilirubin level cohorts. A finer measure later predicted the bilirubin content of each of these groups as low ( < 10 mg / dl ), medium (10 to 15 mg / dl ), and high ( > 15 mg / dl ). Results: Using this hierarchical decision model statistical approach, we quantified the amount of bilirubin in the 20 testing set samples with 82% accuracy. Conclusions: We formulated a biostatistical model in which we automated the spectrometric determination of total bilirubin in the whole blood for patients of neonatal hyperbilirubinemia. (© 2023 The Authors.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|