Quantum Dot-DNA FRET Conjugates for Direct Analysis of Methylphosphonic Acid in Complex Media.
| Autor: | Demers SME; Global Security Directorate, Savannah River National Laboratory, P.O. Box A, Aiken, South Carolina 29808, United States., Kuhne WW; Global Security Directorate, Savannah River National Laboratory, P.O. Box A, Aiken, South Carolina 29808, United States., Swindle AR; Global Security Directorate, Savannah River National Laboratory, P.O. Box A, Aiken, South Carolina 29808, United States., Dick DD; Global Security Directorate, Savannah River National Laboratory, P.O. Box A, Aiken, South Carolina 29808, United States., Coopersmith KJ; Global Security Directorate, Savannah River National Laboratory, P.O. Box A, Aiken, South Carolina 29808, United States. |
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| Jazyk: | angličtina |
| Zdroj: | ACS omega [ACS Omega] 2023 Jun 09; Vol. 8 (25), pp. 23017-23023. Date of Electronic Publication: 2023 Jun 09 (Print Publication: 2023). |
| DOI: | 10.1021/acsomega.3c02173 |
| Abstrakt: | Rapid detection of nerve agents from complex matrices with minimal sample preparation is essential due to their high toxicity and bioavailability. In this work, quantum dots (QDs) were functionalized with oligonucleotide aptamers that specifically targeted a nerve agent metabolite, methylphosphonic acid (MePA). These QD-DNA bioconjugates were covalently linked to quencher molecules to form Förster resonance energy transfer (FRET) donor-acceptor pairs that quantitatively measure the presence of MePA. Using the FRET biosensor, the MePA limit of detection was 743 nM in artificial urine. A decrease in the QD lifetime was measured upon DNA binding and was recovered with MePA. The biosensor's flexible design makes it a strong candidate for the rapid detection of chemical and biological agents for deployable, in-field detectors. Competing Interests: The authors declare the following competing financial interest(s): The authors have filed provisional patent application US: 63/297,361: Biosensor for Simultaneous Chemical, Biological, and Radiological/Nuclear Detection to Battelle Savannah River Alliance, LLC. (© 2023 The Authors. Published by American Chemical Society.) |
| Databáze: | MEDLINE |
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