Identification of Lipoxygenase gene repertoire of Cannabis sativa and functional characterization of CsLOX13 gene.

Autor: Fayaz M; Plant Sciences and Agrotechnology Division, CSIR-Indian Institute of Integrative Medicine Canal Road, Jammu 180001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India., Kundan M; Plant Sciences and Agrotechnology Division, CSIR-Indian Institute of Integrative Medicine Canal Road, Jammu 180001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India., Gani U; Plant Sciences and Agrotechnology Division, CSIR-Indian Institute of Integrative Medicine Canal Road, Jammu 180001, India., Sharma P; Plant Sciences and Agrotechnology Division, CSIR-Indian Institute of Integrative Medicine Canal Road, Jammu 180001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India., Wajid MA; Plant Sciences and Agrotechnology Division, CSIR-Indian Institute of Integrative Medicine Canal Road, Jammu 180001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India., Katoch K; Plant Sciences and Agrotechnology Division, CSIR-Indian Institute of Integrative Medicine Canal Road, Jammu 180001, India., Babu V; Fermentation & Microbial Biotechnology Division, CSIR-Indian Institute of Integrative Medicine Canal Road, Jammu 180001, India., Gairola S; Plant Sciences and Agrotechnology Division, CSIR-Indian Institute of Integrative Medicine Canal Road, Jammu 180001, India., Misra P; Plant Sciences and Agrotechnology Division, CSIR-Indian Institute of Integrative Medicine Canal Road, Jammu 180001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India. Electronic address: pmisra@iiim.res.in.
Jazyk: angličtina
Zdroj: Plant science : an international journal of experimental plant biology [Plant Sci] 2023 Sep; Vol. 334, pp. 111780. Date of Electronic Publication: 2023 Jun 28.
DOI: 10.1016/j.plantsci.2023.111780
Abstrakt: Lipoxygenase (LOX) enzymes play a pivotal role in the biosynthesis of oxylipins. The phyto-oxilipins have been implicated in diverse aspects of plant biology, from regulating plant growth and development to providing tolerance against biotic and abiotic stresses. C. sativa is renowned for its bioactive secondary metabolites, namely cannabinoids. LOX route is assumed to be involved in the biosynthesis of hexanoic acid, which is one of the precursors of cannabinoids of C. sativa. For obvious reasons, the LOX gene family deserves thorough investigation in the C. sativa. Genome-wide analysis revealed the presence of 21 LOX genes in C. sativa, which can be further grouped into 13-LOX and 9-LOX depending upon their phylogeny as well as the enzyme activity. The promoter regions of the CsLOX genes were predicted to contain cis-acting elements involved in phytohormones responsiveness and stress response. The qRT-PCR-based expression analysis of 21 LOX genes revealed their differential expression in different plant parts (root, stem, young leaf, mature leaf, sugar leaf, and female flower). The majority of CsLOX genes displayed preferential expression in the female flower, which is the primary site for the biosynthesis of cannabinoids. The highest LOX activity and expression level of a jasmonate marker gene were reported in the female flowers among all the plant parts. Several CsLOX genes were found to be upregulated by MeJA treatment. Based on the transient expression in Nicotiana benthamiana and the development of stable Nicotiana tabacum transgenic lines, we demonstrate that CsLOX13 encodes functional lipoxygenase and play an important role in the biosynthesis of oxylipins.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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