Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.

Autor: de Jesus ENS; Laboratório de Química dos Produtos Naturais, Universidade do Estado do Pará, Belém, 66087-662, Brazil; Laboratório de Morfofisiologia Aplicada a Saúde, Departamento de Morfologia e Ciências Fisiológicas, Universidade do Estado do Pará, Belém, 66087-662, Brazil. Electronic address: ellen.jesus@aluno.uepa.br., Tavares MS; Laboratório de Morfofisiologia Aplicada a Saúde, Departamento de Morfologia e Ciências Fisiológicas, Universidade do Estado do Pará, Belém, 66087-662, Brazil. Electronic address: mateus.tavares@aluno.uepa.br., Barros PAC; Laboratório de Morfofisiologia Aplicada a Saúde, Departamento de Morfologia e Ciências Fisiológicas, Universidade do Estado do Pará, Belém, 66087-662, Brazil. Electronic address: pedro.iuri@uepa.br., Miller DC; Laboratório de Morfofisiologia Aplicada a Saúde, Departamento de Morfologia e Ciências Fisiológicas, Universidade do Estado do Pará, Belém, 66087-662, Brazil; Programa de Pós- Graduação em Química Medicinal e Modelagem Molecular, Universidade Federal do Pará, Belém, 66075-110, Brazil. Electronic address: daniele.miller@ics.ufpa.br., da Silva PIC; Laboratório de Morfofisiologia Aplicada a Saúde, Departamento de Morfologia e Ciências Fisiológicas, Universidade do Estado do Pará, Belém, 66087-662, Brazil; Programa de Pós-graduação em Farmacologia e Bioquímica, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110, Brazil. Electronic address: pedro.acbarros@aluno.uepa.br., Freitas JJS; Laboratório de Morfofisiologia Aplicada a Saúde, Departamento de Morfologia e Ciências Fisiológicas, Universidade do Estado do Pará, Belém, 66087-662, Brazil. Electronic address: jofre.freitas@uepa.br., de Lima AB; Laboratório de Morfofisiologia Aplicada a Saúde, Departamento de Morfologia e Ciências Fisiológicas, Universidade do Estado do Pará, Belém, 66087-662, Brazil. Electronic address: andersonbentes@uepa.br., Setzer WN; Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT, 84043, USA. Electronic address: wsetzer@chemistry.uah.edu., da Silva JKR; Programa de Pós-graduação em Farmacologia e Bioquímica, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110, Brazil; Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT, 84043, USA. Electronic address: joycekellys@ufpa.br., Figueiredo PLB; Laboratório de Química dos Produtos Naturais, Universidade do Estado do Pará, Belém, 66087-662, Brazil; Programa de Pós-graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará, 66075-110, Brazil. Electronic address: pablo.figueiredo@uepa.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2023 Dec 05; Vol. 317, pp. 116859. Date of Electronic Publication: 2023 Jun 28.
DOI: 10.1016/j.jep.2023.116859
Abstrakt: Ethnopharmacological Relevance: The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
Aim of the Study: This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
Material and Methods: EuEO was obtained by hydrodistillation and analyzed by GC and GC-MS. The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test. Spontaneous locomotor activity was assessed in the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO.
Results: The EuEO displayed a yield of 2.6 ± 0.7%. The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6). The chemical constituents with the highest concentrations were curzerene (33.4 ± 8.5%), caryophyllene oxide (7.6 ± 2.8%), β-elemene (6.5 ± 1.8%), and E-caryophyllene (4.1 ± 0.3%). Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals. EuEO (300 mg/kg) did not cause a reduction in the number of crossings in the open field compared to the vehicle group. The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05). EuEO, at doses of 50, 100, and 200 mg/kg, reduced the number of abdominal writhings by 61.66%, 38.33%, and 33.33%. EuEO did not show increased hot plate test time latency in any of the intervals analyzed. At 200 mg/kg, EuEO decreased paw licking time, with inhibition of 63.43%. In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%. The groups treated with EuEO at doses of 50, 100, and 200 mg/kg showed ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively. Moreover, EuEO inhibited leukocyte recruitment only at a dose of 200 mg/kg. The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
Conclusion: The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity. This work confirms the antinociceptive and anti-inflammatory of this species as the traditional use.
Competing Interests: Declaration of competing interest The authors declare no conflicts of interest.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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