Nephroprotective effects of Acacia senegal against aflatoxicosis via targeting inflammatory and apoptotic signaling pathways.

Autor: Shanab O; Department of Biochemistry, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt. Electronic address: shanab.bio@vet.svu.edu.eg., El-Rayes SM; Department of Chemistry, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt. Electronic address: Samir_elrayes@yahoo.com., Khalil WF; Department of Veterinary Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt. Electronic address: wk@vet.suez.edu.eg., Ahmed N; Department of Chemistry, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt. Electronic address: nohaahmed2552015@gmail.com., Abdelkader A; Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University, Benha 13518, Egypt. Electronic address: afaf.abdelkader@fmed.bu.edu.eg., Aborayah NH; Department of Pharmacology, Faculty of Medicine, Benha University, Benha 13518, Egypt; Department of Pharmacology, Faculty of Medicine, Mutah University, Mutah 61710, Jordan. Electronic address: nashwa.aborayah@fmed.bu.edu.eg., Atwa AM; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Egyptian Russian University, Cairo 11829, Egypt. Electronic address: ahmed-atwa@eru.edu.eg., Mohammed FI; Department of Physiology, Faculty of Medicine for Girls, Al-Azhar University, Cairo 11884, Egypt. Electronic address: fatenebrahem.medg@azhar.edu.eg., Nasr HE; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Benha University, Benha 13518, Egypt. Electronic address: hend.mosalm@fmed.bu.edu.eg., Ibrahim SF; Department of Clinical Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: sfibrahim@pnu.edu.sa., Khattab AM; Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Cairo 11956, Egypt; Department of Clinical Toxicology, Dammam Poison Control Center, MOH, Dammam 32245, Saudi Arabia. Electronic address: ammkhattab@moh.gov.sa., Alsieni M; Department of Clinical Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia. Electronic address: malsieni@kau.edu.sa., Behairy A; Department of Pharmacology, Faculty of Medicine, Benha University, Benha 13518, Egypt. Electronic address: ali.behery@fmed.bu.edu.eg., Fericean L; Department of Biology and Plant Protection, Faculty of Agriculture, University of Life Sciences 'King Michael I' from Timișoara, Calea Aradului 119, CUI 3487181, Romania. Electronic address: mihaelafericean@usab-tm.ro., Mohammed LA; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Benha University, Benha 13518, Egypt. Electronic address: lina.mohamed@fmed.bu.edu.eg., Abdeen A; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt. Electronic address: ahmed.abdeen@fvtm.bu.edu.eg.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2023 Jun 27; Vol. 262, pp. 115194. Date of Electronic Publication: 2023 Jun 27.
DOI: 10.1016/j.ecoenv.2023.115194
Abstrakt: Aflatoxin B 1 (AFB1) is a common environmental pollutant that poses a major hazard to both humans and animals. Acacia senegal (Gum) is well-known for having antioxidant and anti-inflammatory bioactive compounds. Our study aimed to scout the nephroprotective effects of Acacia gum (Gum) against AFB1-induced renal damage. Four groups of rats were designed: Control, Gum (7.5 mg/kg), AFB1 (200 µg/kg b.w) and AFB1-Gum, rats were co-treated with both Gum and AFB1. Gas chromatography-mass spectrometry (GC/MS) analysis was done to determine the phytochemical constituents in Gum. AFB1 triggered profound alterations in kidney function parameters (urea, creatinine, uric acid, and alkaline phosphatase) and renal histological architecture. Additionally, AFB1 exposure evoked up-regulation of mRNA expression levels of inflammatory cytokines, including interleukin-6 (IL-6), tumor necrosis factor α (TNFα), inducible nitric oxide synthase (iNOS), and nuclear factor kB p65 (NF-κB/P65) in renal tissue. The oxidative distress and apoptotic cascade are also instigated by AFB1 intoxication as depicted in down-regulated protein expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) and superoxide dismutase type 1 (SOD1) along with upregulation of cytochrome c (Cyto c), and cleaved Caspase3 (Casp3-17 and 19) in renal tissue. In conclusion, current study obviously confirms the alleviating effects of Gum supplementation against AFB1-induced renal dysfunction, oxidative harm, inflammation, and cell death. These mitigating effects are suggested to be attributed to Gum's antioxidant and anti-inflammatory activities. Our results recommend Gum supplementation as add-on agents to food that might aid in protection from AFB1-induced nephrotoxicity.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Samah F. Ibrahim reports financial support was provided by Princess Nourah bint Abdulrahman University. Liana Fericean reports financial support was provided by University of Life Sciences.
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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