Effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle.

Autor:	McAtee TB; Department of Agricultural Sciences, West Texas A&M University, Canyon, TX, United States.; VERO Program, Texas A&M University, Canyon, TX, United States., Pinnell LJ; VERO Program, Texas A&M University, Canyon, TX, United States., Powledge SA; Department of Agricultural Sciences, West Texas A&M University, Canyon, TX, United States., Wolfe CA; VERO Program, Texas A&M University, Canyon, TX, United States., Morley PS; VERO Program, Texas A&M University, Canyon, TX, United States., Richeson JT; Department of Agricultural Sciences, West Texas A&M University, Canyon, TX, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Jun 13; Vol. 14, pp. 1203498. Date of Electronic Publication: 2023 Jun 13 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1203498
Abstrakt:	Introduction: The objectives of this study were to evaluate the impacts of two modified-live virus (MLV) vaccination protocols and respiratory disease (BRD) occurrence on the microbial community composition of the nasopharynx in feedlot cattle. Methods: The treatment groups included in this randomized controlled trial included: 1) no viral respiratory vaccination (CON), 2) intranasal, trivalent, MLV respiratory vaccine in addition to a parenteral BVDV type I and II vaccine (INT), and 3) parenteral, pentavalent, MLV respiratory vaccination against the same agents (INJ). Calves ( n = 525) arrived in 5 truckload blocks and were stratified by body weight, sex, and presence of a pre-existing identification ear-tag. A total of 600 nasal swab samples were selected for DNA extraction and subsequent 16S rRNA gene sequencing to characterize the microbiome of the upper respiratory tract. Nasal swabs collected on d 28 from healthy cattle were used to evaluate the impact of vaccination on upper respiratory tract (URT) microbial communities. Results: Firmicutes were less abundant in INT calves ( n = 114; P < 0.05) and this difference was attributed to decreased relative abundance (RA) of Mycoplasma spp . ( P = 0.04). Mannheimia and Pasteurella had lower RA in INT ( P < 0.05). The microbiome in healthy animals on d 28 had increased Proteobacteria (largely Moraxella spp.) and decreased Firmicutes (comprised almost exclusively of Mycoplasma spp. ) compared to animals that were treated for or died from BRD ( P < 0.05). Cattle that died had a greater RA of Mycoplasma spp . in their respiratory microbiome on d 0 ( P < 0.02). Richness was similar on d 0 and 28, but diversity increased for all animals on d 28 ( P >0.05). Competing Interests: JR has received research funding and honoraria for consulting and seminar presentations from companies that manufacture and market vaccines for administration in cattle. PM and JR have received in-kind and financial support from commercial entities for studies investigating respiratory disease, microbial ecology, and antimicrobial resistance in cattle. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 McAtee, Pinnell, Powledge, Wolfe, Morley and Richeson.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu