Stereological Evaluation of Rabbit Fetus Liver after Xenotransplantation of Human Wharton's Jelly-Derived Mesenchymal Stromal Cells.

Autor: Khodabandeh Z; Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Rezaeian L; Department of Biology, Faculty of Sciences, Farhangian University, Tehran, Iran., Edalatmanesh MA; Department of Biology, Faculty of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran., Mogheiseh A; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Tanideh N; Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.; Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Dianatpour M; Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.; Department of Human Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Zare S; Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Bordbar H; Histomorphometry and Stereology Research Center, Shiraz University of Medical Science, Shiraz, Iran.; Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran., Baghban N; Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran., Tamadon A; PerciaVista R&D Co. Shiraz, Iran.
Jazyk: angličtina
Zdroj: International journal of organ transplantation medicine [Int J Organ Transplant Med] 2022; Vol. 13 (1), pp. 15-24.
Abstrakt: Background: In-utero xenotransplantation of stem cells in abnormal fetuses effectively treats several genetic illnesses.
Objective: The current research aimed to evaluate structural and morphological alterations in the liver of rabbit fetuses following xenotransplantation of human Wharton's jelly-derived mesenchymal stromal cells (hWJ-MSCs), using a stereological technique.
Methods: All hWJ-MSCs were isolated from the human umbilical cord, and their authenticity was established by flowcytometry and differentiation. At gestational day 14, the rabbits were anesthetized, and hWJ-MSCs were injected into the uteri of 24 fetuses. Twenty-two fetuses were born successfully. Ten rabbit liver specimens were prepared from injected fetuses including; eight rabbits on day three following birth and two rabbits on the 21st post-natal day. The non-injected fetuses were considered positive controls. The livers of the control and hWJ-MSCs-treated rabbits were fixed, processed, stained, and examined through stereological approaches.
Results: In the hWJ-MSCs-treated group, the mean liver weight and volume increased by 42% and 78% compared to the control group. The total volume of the hepatocytes increased by 63%, and that of sinusoids by three folds in the treated rabbits. The total volume of the central veins increased by 70%. The total number corresponding to hepatocytes in the experimental group increased by 112% compared to the rabbits in the control. The total volume of the hepatocyte nuclei in the experimental group increased by 117% compared to the rabbits in the control.
Conclusion: After xenotransplantation of human MSCs, host tissue microenvironments (here, the rabbit liver) were altered and these included quantitative factors corresponding to the liver tissue and hepatocyte morphometric indices.
Competing Interests: None declared.
Databáze: MEDLINE
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