[Protective effect of melatonin against oxygen-induced retinopathy: a study based on the HMGB1/NF-κB/NLRP3 axis].

Autor:	Chu FF; Department of Anatomy, Weifang Medical College, Weifang, Shandong 261053, China; Ophthalmology Center, Affiliated Hospital of Weifang Medical College, Weifang, Shandong 261031, China., Zhao YS, Zhao YZ, Bai C; Department of Anatomy, Weifang Medical College, Weifang, Shandong 261053, China; Ophthalmology Center, Affiliated Hospital of Weifang Medical College, Weifang, Shandong 261031, China., Xiao PL; Department of Anatomy, Weifang Medical College, Weifang, Shandong 261053, China; Ophthalmology Center, Affiliated Hospital of Weifang Medical College, Weifang, Shandong 261031, China., Wang XL, Yu SN; Department of Anatomy, Weifang Medical College, Weifang, Shandong 261053, China; Ophthalmology Center, Affiliated Hospital of Weifang Medical College, Weifang, Shandong 261031, China., Jiang JY; Department of Anatomy, Weifang Medical College, Weifang, Shandong 261053, China; Ophthalmology Center, Affiliated Hospital of Weifang Medical College, Weifang, Shandong 261031, China.
Jazyk:	čínština
Zdroj:	Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics [Zhongguo Dang Dai Er Ke Za Zhi] 2023 Jun 15; Vol. 25 (6), pp. 645-652.
DOI:	10.7499/j.issn.1008-8830.2301036
Abstrakt:	Objectives: To study the protective effect of melatonin (Mel) against oxygen-induced retinopathy (OIR) in neonatal mice and the role of the HMGB1/NF-κB/NLRP3 axis. Methods: Neonatal C57BL/6J mice, aged 7 days, were randomly divided into a control group, a model group (OIR group), and a Mel treatment group (OIR+Mel group), with 9 mice in each group. The hyperoxia induction method was used to establish a model of OIR. Hematoxylin and eosin staining and retinal flat-mount preparation were used to observe retinal structure and neovascularization. Immunofluorescent staining was used to measure the expression of proteins and inflammatory factors associated with the HMGB1/NF-κB/NLRP3 axis and lymphocyte antigen 6G. Colorimetry was used to measure the activity of myeloperoxidase. Results: The OIR group had destruction of retinal structure with a large perfusion-free area and neovascularization, while the OIR+Mel group had improvement in destruction of retinal structure with reductions in neovascularization and perfusion-free area. Compared with the control group, the OIR group had significant increases in the expression of proteins and inflammatory factors associated with the HMGB1/NF-κB/NLRP3 axis, the expression of lymphocyte antigen 6G, and the activity of myeloperoxidase ( P <0.05). Compared with the OIR group, the OIR+Mel group had significant reductions in the above indices ( P <0.05). Compared with the control group, the OIR group had significant reductions in the expression of melatonin receptors in the retina ( P <0.05). Compared with the OIR group, the OIR+Mel group had significant increases in the expression of melatonin receptors ( P <0.05). Conclusions: Mel can alleviate OIR-induced retinal damage in neonatal mice by inhibiting the HMGB1/NF-κB/NLRP3 axis and may exert an effect through the melatonin receptor pathway.
Databáze:	MEDLINE
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