Overexpression of the UDP-glycosyltransferase UGT34A23 confers resistance to the diamide insecticide chlorantraniliprole in the tomato leafminer, Tuta absoluta.

Autor: Grant C; Centre for Ecology and Conservation, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall, UK., Singh KS; Centre for Ecology and Conservation, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall, UK., Hayward A; Centre for Ecology and Conservation, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall, UK., Hunt BJ; Centre for Ecology and Conservation, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall, UK., Troczka BJ; Centre for Ecology and Conservation, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall, UK., Pym A; Centre for Ecology and Conservation, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall, UK., Ahn SJ; Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, MS, United States., Zeng B; Centre for Ecology and Conservation, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall, UK; College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Jiangsu, China., Gao CF; College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Jiangsu, China., Leroux A; Syngenta Crop Protection, Werk Stein, Schaffhauserstrasse, Stein, CH4332, Switzerland., Daum E; Syngenta Crop Protection, Werk Stein, Schaffhauserstrasse, Stein, CH4332, Switzerland., Süess P; Syngenta Crop Protection, Werk Stein, Schaffhauserstrasse, Stein, CH4332, Switzerland; Department of Zoology, Stockholm University, 11418, Stockholm, Sweden., Souza D; Syngenta Crop Protection, Werk Stein, Schaffhauserstrasse, Stein, CH4332, Switzerland., Elias J; Syngenta Crop Protection, Werk Stein, Schaffhauserstrasse, Stein, CH4332, Switzerland., Ffrench-Constant RH; Centre for Ecology and Conservation, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall, UK., Vontas J; Institute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, Crete, Greece; Department of Crop Science, Agricultural University of Athens, Athens, Greece., Roditakis E; Department of Agriculture, Hellenic Mediterranean University, Heraklion, Crete, Greece., Bielza P; Departamento de Producción Vegetal, Universidad Politécnica de Cartagena, Paseo Alfonso XIII 48, 30203, Spain., Zimmer CT; Syngenta Crop Protection, Werk Stein, Schaffhauserstrasse, Stein, CH4332, Switzerland. Electronic address: christoph.zimmer@syngenta.com., Bass C; Centre for Ecology and Conservation, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall, UK. Electronic address: c.bass@exeter.ac.uk.
Jazyk: angličtina
Zdroj: Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2023 Aug; Vol. 159, pp. 103983. Date of Electronic Publication: 2023 Jun 26.
DOI: 10.1016/j.ibmb.2023.103983
Abstrakt: The tomato leafminer, Tuta absoluta, is an invasive crop pest that has evolved resistance to many of the insecticides used for its control. To facilitate the investigation of the underpinning mechanisms of resistance in this species we generated a contiguous genome assembly using long-read sequencing data. We leveraged this genomic resource to investigate the genetic basis of resistance to the diamide insecticide chlorantraniliprole in Spanish strains of T. absoluta that exhibit high levels of resistance to this insecticide. Transcriptomic analyses revealed that, in these strains, resistance is not associated with previously reported target-site mutations in the diamide target-site, the ryanodine receptor, but rather is associated with the marked overexpression (20- to >100-fold) of a gene encoding a UDP-glycosyltransferase (UGT). Functional expression of this UGT, UGT34A23, via ectopic expression in Drosophila melanogaster demonstrated that it confers strong and significant resistance in vivo. The genomic resources generated in this study provide a powerful resource for further research on T. absoluta. Our findings on the mechanisms underpinning resistance to chlorantraniliprole will inform the development of sustainable management strategies for this important pest.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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