Morphologic design of nanogold carriers for a carbonic anhydrase inhibitor: Effect on ocular retention and intraocular pressure.

Autor: Eissa NG; Department of Pharmaceutics, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt; Badr University in Cairo Research Center and School of Biotechnology, Badr University in Cairo, Badr City, Cairo 11829, Egypt., Eldehna WM; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh 33516, Egypt. Electronic address: wagdy2000@gmail.com., Abdelazim EB; Badr University in Cairo Research Center and School of Biotechnology, Badr University in Cairo, Badr City, Cairo 11829, Egypt., Eissa RA; Badr University in Cairo Research Center and School of Biotechnology, Badr University in Cairo, Badr City, Cairo 11829, Egypt., Mohamed HH; Badr University in Cairo Research Center and School of Biotechnology, Badr University in Cairo, Badr City, Cairo 11829, Egypt., Diab NH; Pharmaceutics Department, Faculty of Pharmacy, Sphinx University, New Assiut City, Assiut, Egypt., El Hassab MA; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Salman International University (KSIU), South Sinai, Egypt., Elkaeed EB; Department of Pharmaceutical Sciences, College of Pharmacy, AlMaarefa University, Riyadh 13713, Saudi Arabia; Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt., Elsayed ZM; Scientific Research and Innovation Support Unit, Faculty of Pharmacy, Kafrelsheikh Uinversity, Kafrelsheikh, Egypt., Sabet MA; Department of Microbiology and Immunology, Faculty of Pharmacy, Sphinx University, New-Assiut 71684, Egypt., Bakr MH; Department of Histology and Cell Biology, Faculty of Medicine, Assiut University, Assiut, Egypt., Aboelela A; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sphinx University, Assiut 71515, Egypt., Abdelshafi NA; Department of Pharmaceutical Analytical Chemistry, School of Pharmacy, Badr University in Cairo, Badr City, Cairo 11829, Egypt., Kamoun EA; Polymeric Materials Research Dep., Advanced Technology and New Materials Research Institute, the City of Scientific Research and Technological Applications (SRTA-City), Alexandria 21934, Egypt; Nanotechnology Research Centre (NTRC), The British University in Egypt (BUE), El Sherouk City, Suez Desert Road, Cairo 1183, Egypt., Supuran CT; Department of NEUROFARBA, Section of Pharmaceutical and Nutraceutical Sciences, University of Florence, Polo Scientifico, Via U. Schiff 6, 50019, Sesto Fiorentino, Firenze, Italy., Elsabahy M; Badr University in Cairo Research Center and School of Biotechnology, Badr University in Cairo, Badr City, Cairo 11829, Egypt; Department of Chemistry, Texas A&M University, College Station, TX 77842, USA. Electronic address: mahmoud.elsabahy@chem.tamu.edu., Allam AA; Pharmaceutics Department, Faculty of Pharmacy, Sphinx University, New Assiut City, Assiut, Egypt; Pharmaceutics Department, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2023 Jul 25; Vol. 642, pp. 123161. Date of Electronic Publication: 2023 Jun 26.
DOI: 10.1016/j.ijpharm.2023.123161
Abstrakt: Morphologic design of nanomaterials for a diversity of biomedical applications is of increasing interest. The aim of the current study is to construct therapeutic gold nanoparticles of different morphologies and investigate their effect on ocular retention and intraocular pressure in a glaucoma rabbit model. Poly(lactic-co-glycolic acid) (PLGA)-coated nanorods and nanospheres have been synthesized and loaded with carbonic anhydrase inhibitor (CAI), and characterized in vitro for their size, zeta potential and encapsulation efficiency. Nanosized PLGA-coated gold nanoparticles of both morphologies demonstrated high entrapment efficiency (˃ 98%) for the synthesized CAI and the encapsulation of the drug into the developed nanoparticles was confirmed via Fourier transform-infrared spectroscopy. In vivo studies revealed a significant reduction in intraocular pressure upon instillation of drug-loaded nanogold formulations compared to the marketed eye drops. Spherical nanogolds exhibited a superior efficacy compared to the rod-shaped counterparts, probably due to the enhanced ocular retention of spherical nanogolds within collagen fibers of the stroma, as illustrated by transmission electron microscopy imaging. Normal histological appearance was observed for the cornea and retina of the eyes treated with spherical drug-loaded nanogolds. Hence, incorporation of a molecularly-designed CAI into nanogold of tailored morphology may provide a promising strategy for management of glaucoma.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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