Determination of antibiotics by amperometry using nortropine N-oxyl, a highly active nitroxyl radical.

Autor: Ono T; School of Pharmaceutical Sciences, Ohu University, 31-1 Misumido, Tomita-machi, Koriyama, Fukushima, 963-8611, Japan. t-ono@pha.ohu-u.ac.jp., Sato F; Faculty of Pharmaceutical Science, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba, Sendai, Miyagi, 981-8558, Japan., Kumano M; Faculty of Pharmaceutical Science, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba, Sendai, Miyagi, 981-8558, Japan., Komatsu S; Faculty of Pharmaceutical Science, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba, Sendai, Miyagi, 981-8558, Japan., Sugiyama K; Faculty of Pharmaceutical Science, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba, Sendai, Miyagi, 981-8558, Japan., Watanabe K; Faculty of Pharmaceutical Science, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba, Sendai, Miyagi, 981-8558, Japan., Yoshida K; School of Pharmaceutical Sciences, Ohu University, 31-1 Misumido, Tomita-machi, Koriyama, Fukushima, 963-8611, Japan., Sasano Y; Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, Sendai, 980-8578, Japan., Fujimura T; Faculty of Pharmaceutical Science, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba, Sendai, Miyagi, 981-8558, Japan., Iwabuchi Y; Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, Sendai, 980-8578, Japan., Kashiwagi Y; School of Pharmaceutical Sciences, Ohu University, 31-1 Misumido, Tomita-machi, Koriyama, Fukushima, 963-8611, Japan., Sato K; Faculty of Pharmaceutical Science, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba, Sendai, Miyagi, 981-8558, Japan. satok@tohoku-mpu.ac.jp.
Jazyk: angličtina
Zdroj: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2023 Oct; Vol. 39 (10), pp. 1771-1775. Date of Electronic Publication: 2023 Jun 28.
DOI: 10.1007/s44211-023-00388-4
Abstrakt: Nitroxyl radical compounds oxidize hydroxy groups and some amino groups upon application of an electric potential. The resulting anodic current depends on the concentration of these functional groups in solution. Thus, it is possible to quantify compounds containing these functional groups by electrochemical methods. Cyclic voltammetry has been used to evaluate the catalytic activity of nitroxyl radicals, and the ability of such radicals to sense biological and other compounds. In this study, we evaluated a method for quantifying compounds using constant-potential electrolysis (amperometry) of nitroxyl radicals for application in flow injection analysis and high-performance liquid chromatography as an electrochemical detector. When amperometry was performed using 2,2,6,6-tetramethylpiperidine 1-oxyl, a common nitroxyl radical compound, little change was observed even with 100 mM glucose due to its low reactivity in neutral aqueous solutions. In contrast, 2-azaadamantane N-oxyl and nortropine N-oxyl, which are highly active nitroxyl radicals, showed a concentration-dependent response in neutral aqueous solution. Responses of 33.8 and 125.9 μA, respectively, were observed. By recognition of hydroxy and amino groups, we have succeeded in the electrochemical detection of some drugs by amperometry. Streptomycin, an aminoglycoside antibiotic, was quantifiable in the range of 30-1000 µM.
(© 2023. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry.)
Databáze: MEDLINE
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