Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1.

Autor:	Zannotti M; Chemistry Interdisciplinary Project, School of Science and Technology, Chemistry Division, University of Camerino 62032 Camerino Italy marco.zannotti@unicam.it rita.giovannetti@unicam.it.; IridES s.r.l. Via Via Gentile III da Varano n° 1 62032 Camerino Italy., Ramasamy KP; Department of Ecology and Environmental Science, Umeå University Umeå 901 87 Sweden., Loggi V; Chemistry Interdisciplinary Project, School of Science and Technology, Chemistry Division, University of Camerino 62032 Camerino Italy marco.zannotti@unicam.it rita.giovannetti@unicam.it., Vassallo A; School of Biosciences and Veterinary Medicine, Biosciences and Biotechnology Division, University of Camerino 62032 Camerino Italy., Pucciarelli S; School of Biosciences and Veterinary Medicine, Biosciences and Biotechnology Division, University of Camerino 62032 Camerino Italy.; IridES s.r.l. Via Via Gentile III da Varano n° 1 62032 Camerino Italy., Giovannetti R; Chemistry Interdisciplinary Project, School of Science and Technology, Chemistry Division, University of Camerino 62032 Camerino Italy marco.zannotti@unicam.it rita.giovannetti@unicam.it.; IridES s.r.l. Via Via Gentile III da Varano n° 1 62032 Camerino Italy.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2023 Jun 26; Vol. 13 (28), pp. 19276-19285. Date of Electronic Publication: 2023 Jun 26 (Print Publication: 2023).
DOI:	10.1039/d3ra02536e
Abstrakt:	One of the most concerning environmental problems is represented by petroleum and its derivatives causing contamination of aquatic and underground environments. In this work, the degradation treatment of diesel using Antarctic bacteria is proposed. Marinomonas sp. ef1 is a bacterial strain isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii . Its potential in the degradation of hydrocarbons commonly present in diesel oil were studied. The bacterial growth was evaluated in culturing conditions that resembled the marine environment with 1% (v/v) of either diesel or biodiesel added; in both cases, Marinomonas sp. ef1 was able to grow. The chemical oxygen demand measured after the incubation of bacteria with diesel decreased, demonstrating the ability of bacteria to use diesel hydrocarbons as a carbon source and degrade them. The metabolic potential of Marinomonas to degrade aromatic compounds was supported by the identification in the genome of sequences encoding various enzymes involved in benzene and naphthalene degradation. Moreover, in the presence of biodiesel, a fluorescent yellow pigment was produced; this was isolated, purified and characterized by UV-vis and fluorescence spectroscopy, leading to its identification as a pyoverdine. These results suggest that Marinomonas sp. ef1 can be used in hydrocarbon bioremediation and in the transformation of these pollutants in molecules of interest. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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