Transinteractome analysis reveals distinct niche requirements for isotype-based plasma cell subsets in the bone marrow.

Autor:	Bonaud A; Université Paris Cité, Institut de Recherche Saint-Louis, INSERM U1160, Paris, France.; OPALE Carnot Institute, Hôpital St-Louis, Paris, France., Larraufie P; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France., Khamyath M; Université Paris Cité, Institut de Recherche Saint-Louis, INSERM U1160, Paris, France.; OPALE Carnot Institute, Hôpital St-Louis, Paris, France., Szachnowski U; Université Paris-Saclay, INSERM, Inflammation, Microbiome and Immunosurveillance, Clamart, France.; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Flint SM; Université Paris-Saclay, INSERM, Inflammation, Microbiome and Immunosurveillance, Clamart, France., Brunel-Meunier N; Sorbonne Université, INSERM, Centre de Recherche Saint-Antoine (CRSA), AP-HP, Saint-Antoine Hospital, Paris, France., Delhommeau F; Sorbonne Université, INSERM, Centre de Recherche Saint-Antoine (CRSA), AP-HP, Saint-Antoine Hospital, Paris, France., Munier A; Sorbonne Université-INSERM UMRS_938, Centre de Recherche Saint-Antoine (CRSA), Plateforme de Cytométrie CISA, Paris, France., Lönnberg T; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, UK.; Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK., Toffano-Nioche C; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Gautheret D; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France., Balabanian K; Université Paris Cité, Institut de Recherche Saint-Louis, INSERM U1160, Paris, France.; OPALE Carnot Institute, Hôpital St-Louis, Paris, France., Espéli M; Université Paris Cité, Institut de Recherche Saint-Louis, INSERM U1160, Paris, France.; OPALE Carnot Institute, Hôpital St-Louis, Paris, France.
Jazyk:	angličtina
Zdroj:	European journal of immunology [Eur J Immunol] 2023 Sep; Vol. 53 (9), pp. e2250334. Date of Electronic Publication: 2023 Jun 28.
DOI:	10.1002/eji.202250334
Abstrakt:	Bone marrow (BM) long-lived plasma cells (PCs) are essential for long-term protection against infection, and their persistence within this organ relies on interactions with Cxcl12-expressing stromal cells that are still not clearly identified. Here, using single cell RNAseq and in silico transinteractome analyses, we identified Leptin receptor positive (LepR + ) mesenchymal cells as the stromal cell subset most likely to interact with PCs within the BM. Moreover, we demonstrated that depending on the isotype they express, PCs may use different sets of integrins and adhesion molecules to interact with these stromal cells. Altogether, our results constitute an unprecedented characterization of PC subset stromal niches and open new avenues for the specific targeting of BM PCs based on their isotype. (© 2023 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.)
Databáze:	MEDLINE
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