Transforming an esterase into an enantioselective catecholase through bioconjugation of a versatile metal-chelating inhibitor.

Autor: Fernandez-Lopez L; Instituto de Catalisis y Petroleoquimica (ICP), CSIC, Madrid 28049, Spain. mferrer@icp.csic.es., Cea-Rama I; Instituto de Quimica Fisica Rocasolano (IQFR), CSIC, Madrid 28006, Spain., Alvarez-Malmagro J; Instituto de Catalisis y Petroleoquimica (ICP), CSIC, Madrid 28049, Spain. mferrer@icp.csic.es., Ressmann AK; Eucodis Bioscience GMBH, Wien 1030, Austria., Gonzalez-Alfonso JL; Instituto de Catalisis y Petroleoquimica (ICP), CSIC, Madrid 28049, Spain. mferrer@icp.csic.es., Coscolín C; Instituto de Catalisis y Petroleoquimica (ICP), CSIC, Madrid 28049, Spain. mferrer@icp.csic.es., Shahgaldian P; Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz 4132, Switzerland., Plou FJ; Instituto de Catalisis y Petroleoquimica (ICP), CSIC, Madrid 28049, Spain. mferrer@icp.csic.es., Modregger J; Eucodis Bioscience GMBH, Wien 1030, Austria., Pita M; Instituto de Catalisis y Petroleoquimica (ICP), CSIC, Madrid 28049, Spain. mferrer@icp.csic.es., Sanz-Aparicio J; Instituto de Quimica Fisica Rocasolano (IQFR), CSIC, Madrid 28006, Spain., Ferrer M; Instituto de Catalisis y Petroleoquimica (ICP), CSIC, Madrid 28049, Spain. mferrer@icp.csic.es.
Jazyk: angličtina
Zdroj: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2023 Aug 01; Vol. 59 (62), pp. 9469-9472. Date of Electronic Publication: 2023 Aug 01.
DOI: 10.1039/d3cc01946b
Abstrakt: Metal complexes introduced into protein scaffolds can generate versatile biomimetic catalysts endowed with a variety of catalytic properties. Here, we synthesized and covalently bound a bipyridinyl derivative to the active centre of an esterase to generate a biomimetic catalyst that shows catecholase activity and enantioselective catalytic oxidation of (+)-catechin.
Databáze: MEDLINE
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