GFP-related chromophores: photoisomerization, thermal reversion, and DNA labelling.

Autor: Faillace MS; INFIQC-CONICET-Dpto. de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, X5000HUA, Argentina. walter.pelaez@unc.edu.ar.; Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA., Dolgopolova EA; Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA., Ceballos NM; INFIQC-CONICET-Dpto. de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, X5000HUA, Argentina. walter.pelaez@unc.edu.ar., Ruiz Pereyra EN; INFIQC-CONICET-Dpto. de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, X5000HUA, Argentina. walter.pelaez@unc.edu.ar., Lanfri L; INFIQC-CONICET-Dpto. de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, X5000HUA, Argentina. walter.pelaez@unc.edu.ar., Argüello GA; INFIQC-CONICET-Dpto. de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, X5000HUA, Argentina. walter.pelaez@unc.edu.ar., Burgos Paci M; INFIQC-CONICET-Dpto. de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, X5000HUA, Argentina. walter.pelaez@unc.edu.ar., Shustova NB; Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA., Peláez WJ; INFIQC-CONICET-Dpto. de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, X5000HUA, Argentina. walter.pelaez@unc.edu.ar.
Jazyk: angličtina
Zdroj: Physical chemistry chemical physics : PCCP [Phys Chem Chem Phys] 2023 Jul 12; Vol. 25 (27), pp. 17943-17951. Date of Electronic Publication: 2023 Jul 12.
DOI: 10.1039/d3cp01655b
Abstrakt: Due to the pronounced effect of the confined environment on the photochemical properties of 4-hydroxybenzylidene imidazolinone (HBI), a GFP-related chromophore, imidazolidinone and imidazothiazolone analogues have been studied as fluorescent probes. Their photoisomerization and their thermal reversion were studied under 365-nm-irradiation, resulting in observation of an enthalpy-entropy compensation effect. Theoretical studies were carried out to shed light on the thermal reversion mechanism. Moreover, photophysical studies of benzylidene imidazothiazolone in the presence of dsDNA revealed fluorescence enhancement. The prepared compounds could be considered as a valuable tool for the detailed investigation of physicochemical, biochemical, or biological systems.
Databáze: MEDLINE
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