| Autor: |
Vu TT; Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea., Yadav S; Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea., Reddy OS; Department of Display Engineering, Pukyong National University, Busan 48513, Republic of Korea., Jo SH; Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea., Joo SB; Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea., Kim BK; Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea., Park EJ; Institute of Materials Research and Engineering, Agency for Science, Technology and Research, Singapore 138634, Singapore., Park SH; Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea., Lim KT; Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea.; Department of Display Engineering, Pukyong National University, Busan 48513, Republic of Korea. |
| Abstrakt: |
Selective delivery of anticancer drug molecules to the tumor site enhances local drug dosages, which leads to the death of cancer cells while simultaneously minimizing the negative effects of chemotherapy on other tissues, thereby improving the patient's quality of life. To address this need, we developed reduction-responsive chitosan-based injectable hydrogels via the inverse electron demand Diels-Alder reaction between tetrazine groups of disulfide-based cross-linkers and norbornene groups of chitosan derivatives, which were applied to the controlled delivery of doxorubicin (DOX). The swelling ratio, gelation time (90-500 s), mechanical strength (G'~350-850 Pa), network morphology, and drug-loading efficiency (≥92%) of developed hydrogels were investigated. The in vitro release studies of the DOX-loaded hydrogels were performed at pH 7.4 and 5.0 with and without DTT (10 mM). The biocompatibility of pure hydrogel and the in vitro anticancer activity of DOX-loaded hydrogels were demonstrated via MTT assay on HEK-293 and HT-29 cancer cell lines, respectively. |
