| Autor: |
Mzee T; Ifakara Health Institute, Bagamoyo Branch, Bagamoyo P.O. Box 74, Tanzania.; Department of Molecular Biology and Biotechnology, University of Dar es Salaam, Dar es Salaam P.O. Box 35179, Tanzania., Kumburu H; Kilimanjaro Clinical Research Institute, Moshi P.O. Box 2236, Tanzania., Kazimoto T; Ifakara Health Institute, Bagamoyo Branch, Bagamoyo P.O. Box 74, Tanzania., Leekitcharoenphon P; Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, Kemitorvet, DK 2800 Kgs. Lyngby, Denmark., van Zwetselaar M; Kilimanjaro Clinical Research Institute, Moshi P.O. Box 2236, Tanzania., Masalu R; Department of Molecular Biology and Biotechnology, University of Dar es Salaam, Dar es Salaam P.O. Box 35179, Tanzania., Mlaganile T; Ifakara Health Institute, Bagamoyo Branch, Bagamoyo P.O. Box 74, Tanzania., Sonda T; Kilimanjaro Clinical Research Institute, Moshi P.O. Box 2236, Tanzania., Wadugu B; Kilimanjaro Clinical Research Institute, Moshi P.O. Box 2236, Tanzania., Mushi I; Kilimanjaro Clinical Research Institute, Moshi P.O. Box 2236, Tanzania., Aarestrup FM; Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, Kemitorvet, DK 2800 Kgs. Lyngby, Denmark., Matee M; Department of Microbiology and Immunology, School of Medicine, Muhimbili University of Health and Allied Sciences, Dar es Salaam P.O. Box 65001, Tanzania. |
| Abstrakt: |
Staphylococcus aureus is a common cause of infection in humans and animals, including bovine mastitis, globally. The objective of this study was to genetically characterize a collection of S. aureus isolates recovered from milk and nasal swabs from humans with and without animal contact (bovine = 43, human = 12). Using whole genome sequencing (NextSeq550), isolates were sequence typed, screened for antimicrobial resistance and virulence genes and examined for possible inter-species host transmission. Multi locus sequence typing (MLST) and single nucleotide polymorphism (SNP)-based phylogeny revealed 14 different sequence types, including the following six novel sequence types: ST7840, 7841, 7845, 7846, 7847, and 7848. The SNP tree confirmed that MLST clustering occurred most commonly within CC97, CC5477, and CC152. ResFinder analysis revealed five common antibiotic resistance genes, namely tet (K), blaZ , dfrG , erm ©, and str , encoding for different antibiotics. mecA was discovered in one human isolate only. Multidrug resistance was observed in 25% of the isolates, predominantly in CC152 (7/8) and CC121 (3/4). Known bovine S. aureus (CC97) were collected in humans and known human S. aureus lineages (CC152) were collected in cattle; additionally, when these were compared to bovine-isolated CC97 and human-isolated CC152, respectively, no genetic distinction could be observed. This is suggestive of inter-host transmission and supports the need for surveillance of the human-animal interface. |
