| Autor: |
Monyama MC; Department of Life and Consumer Sciences, University of South Africa, Florida 1710, South Africa.; Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2520, South Africa., Taioe OM; Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2520, South Africa.; Epidemiology, Parasites and Vectors, Agricultural Research Council-Onderstepoort Veterinary Research, Pretoria 0110, South Africa., Nkhebenyane JS; Department of Life Sciences, Central University of Technology, Bloemfontein 9300, South Africa., van Wyk D; Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2520, South Africa., Ramatla T; Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2520, South Africa., Thekisoe OMM; Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2520, South Africa. |
| Jazyk: |
angličtina |
| Zdroj: |
Microorganisms [Microorganisms] 2023 May 30; Vol. 11 (6). Date of Electronic Publication: 2023 May 30. |
| DOI: |
10.3390/microorganisms11061440 |
| Abstrakt: |
Houseflies are alleged reservoirs as well as vectors of human and animal pathogens, including bacteria, because they frequently have contact with animal excreta and decaying organic substances. The rapid adaptation process of ingested microbes in the insect gut may involve gene transfer, including antibiotic resistance determinants among different bacterial strains. Six hundred and fifty-seven (n = 657) houseflies were collected from hospices and were identified morphologically and genetically using the 16S rRNA , CO1 , and ITS2 barcoding genes. This study also characterized the bacterial communities harboured by the captured houseflies using 16S rRNA metabarcoding on the next-generation sequencing (NGS) platform and further sought to detect antibiotic resistance traits by using gene-specific PCR assays. Generated sequences for the targeted gene fragments matched with Musca domestica and all the sequences were deposited to the GenBank database. The 16S rRNA metabarcoding analysis revealed that the most abundant phyla detected with variable abundance observed among all the houseflies were Proteobacteria , followed by Firmicutes , and Bacteroidetes . Furthermore, the NGS data revealed the presence of multiple bacterial genera, including Providencia , Enterobacter , Dysgonomonas , Escherichia-Shigella , Klebsiella , Pseudomonas , and Streptococcus , which are known to harbour potentially pathogenic species of animals and humans. Antibiotic resistance genes detected from the housefly DNA in this study included ermB , tetA , blaSHV , and blaTEM . Moreover, these genes are associated with resistance to erythromycin, tetracycline, and beta-lactams antibiotics, respectively. The presence of bacterial pathogens and the detection of antibiotic resistance genes from the houseflies collected from the hospices indicates the possible health risk to patients in hospices and the surrounding community. Therefore, it is imperative to keep high standards of hygiene, food preparation, safety, and control of houseflies in hospices. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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