Saliva Decreases Sucrose-Induced Cariogenicity in an Experimental Biological Caries Model.

Autor:	Giacaman RA; Cariology Unit, Department of Oral Rehabilitation, Faculty of Dentistry, University of Talca, Talca 3462227, Chile.; Interuniversity Center for Healthy Aging, Consortium of Chilean State Universities, Chilecito 03825, Chile., Umaña R; Cariology Unit, Department of Oral Rehabilitation, Faculty of Dentistry, University of Talca, Talca 3462227, Chile., Nuñez MJ; Cariology Unit, Department of Oral Rehabilitation, Faculty of Dentistry, University of Talca, Talca 3462227, Chile., Díaz-Garrido N; Cariology Unit, Department of Oral Rehabilitation, Faculty of Dentistry, University of Talca, Talca 3462227, Chile., Echeverría C; Cariology Unit, Department of Oral Rehabilitation, Faculty of Dentistry, University of Talca, Talca 3462227, Chile., García-Manríquez N; Cariology Unit, Department of Oral Rehabilitation, Faculty of Dentistry, University of Talca, Talca 3462227, Chile., Mira A; Genomics and Health Department, Foundation for the Promotion of Health and Biomedical Research (FISABIO), 46020 Valencia, Spain., Fernández CE; Cariology Unit, Department of Oral Rehabilitation, Faculty of Dentistry, University of Talca, Talca 3462227, Chile., Gambetta-Tessini K; Cariology Unit, Department of Oral Rehabilitation, Faculty of Dentistry, University of Talca, Talca 3462227, Chile., Lozano CP; Laboratory of Biochemistry and Oral Biology, Institute for Research in Dental Sciences, Faculty of Dentistry, University of Chile, Santiago 8330111, Chile.
Jazyk:	angličtina
Zdroj:	Microorganisms [Microorganisms] 2023 May 29; Vol. 11 (6). Date of Electronic Publication: 2023 May 29.
DOI:	10.3390/microorganisms11061426
Abstrakt:	Objective: Whether a minimum quantity of saliva inhibit the caries process remains uncertain. This study aimed to investigate the impact of saliva dilutions on an in vitro caries model using Streptococcus mutans ( S. mutans ) biofilms. Methods: S. mutans biofilms were cultivated on enamel and root dentin slabs, in culture media containing different proportions of saliva ( v / v ): 0%, 5%, 10%, 25%, 50%, 75%, and 100% saliva, and exposed to a 10% sucrose solution (5 min, 3x/day), with appropriate controls. After 5 (enamel) and 4 (dentin) days, demineralization, biomass, viable bacteria, and polysaccharide formation were analyzed. The acidogenicity of the spent media was monitored overtime. Each assay was performed in triplicate across two independent experiments (n = 6). Results: In both enamel and dentin, an inverse relationship was observed between acidogenicity, demineralization, and the proportion of saliva. Even small quantities of saliva incorporated into the media led to a noticeable reduction in enamel and dentin demineralization. Saliva presence resulted in significant reductions in biomass, viable S. mutans cells, and polysaccharides, with the effects being concentration-dependent for both tissues. Conclusions: High quantities of saliva can almost completely inhibit sucrose-induced cariogenicity, while even small amounts exhibit a dose-dependent caries-protective effect.
Databáze:	MEDLINE
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