In-Depth Analysis of the Pancreatic Extracellular Matrix during Development for Next-Generation Tissue Engineering.

Autor: Glorieux L; Cell Biology Unit, de Duve Institute, UCLouvain, 1200 Brussels, Belgium., Vandooren L; Cell Biology Unit, de Duve Institute, UCLouvain, 1200 Brussels, Belgium., Derclaye S; Nanobiophysics Lab, Louvain Institute of Biomolecular Science and Technology, UCLouvain, 1348 Louvain-la-Neuve, Belgium., Pyr Dit Ruys S; Louvain Drug Research Institute, UCLouvain, 1200 Brussels, Belgium., Oncina-Gil P; Nanobiophysics Lab, Louvain Institute of Biomolecular Science and Technology, UCLouvain, 1348 Louvain-la-Neuve, Belgium., Salowka A; Centre for Gene Therapy and Regenerative Medicine, King's College London, Great Maze Pond, London SE1 9RT, UK., Herinckx G; de Duve Institute and MASSPROT Platform, UCLouvain, 1200 Brussels, Belgium., Aajja E; Cell Biology Unit, de Duve Institute, UCLouvain, 1200 Brussels, Belgium., Lemoine P; Cell Biology Unit, de Duve Institute, UCLouvain, 1200 Brussels, Belgium., Spourquet C; Cell Biology Unit, de Duve Institute, UCLouvain, 1200 Brussels, Belgium., Lefort H; Cell Biology Unit, de Duve Institute, UCLouvain, 1200 Brussels, Belgium., Henriet P; Cell Biology Unit, de Duve Institute, UCLouvain, 1200 Brussels, Belgium., Tyteca D; Cell Biology Unit, de Duve Institute, UCLouvain, 1200 Brussels, Belgium., Spagnoli FM; Centre for Gene Therapy and Regenerative Medicine, King's College London, Great Maze Pond, London SE1 9RT, UK., Alsteens D; Nanobiophysics Lab, Louvain Institute of Biomolecular Science and Technology, UCLouvain, 1348 Louvain-la-Neuve, Belgium., Vertommen D; de Duve Institute and MASSPROT Platform, UCLouvain, 1200 Brussels, Belgium., Pierreux CE; Cell Biology Unit, de Duve Institute, UCLouvain, 1200 Brussels, Belgium.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Jun 17; Vol. 24 (12). Date of Electronic Publication: 2023 Jun 17.
DOI: 10.3390/ijms241210268
Abstrakt: The pancreas is a complex organ consisting of differentiated cells and extracellular matrix (ECM) organized adequately to enable its endocrine and exocrine functions. Although much is known about the intrinsic factors that control pancreas development, very few studies have focused on the microenvironment surrounding pancreatic cells. This environment is composed of various cells and ECM components, which play a critical role in maintaining tissue organization and homeostasis. In this study, we applied mass spectrometry to identify and quantify the ECM composition of the developing pancreas at the embryonic (E) day 14.5 and postnatal (P) day 1 stages. Our proteomic analysis identified 160 ECM proteins that displayed a dynamic expression profile with a shift in collagens and proteoglycans. Furthermore, we used atomic force microscopy to measure the biomechanical properties and found that the pancreatic ECM was soft (≤400 Pa) with no significant change during pancreas maturation. Lastly, we optimized a decellularization protocol for P1 pancreatic tissues, incorporating a preliminary crosslinking step, which effectively preserved the 3D organization of the ECM. The resulting ECM scaffold proved suitable for recellularization studies. Our findings provide insights into the composition and biomechanics of the pancreatic embryonic and perinatal ECM, offering a foundation for future studies investigating the dynamic interactions between the ECM and pancreatic cells.
Databáze: MEDLINE
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