USP15-USP7 Axis and UBE2T Differential Expression May Predict Pathogenesis and Poor Prognosis in De Novo Myelodysplastic Neoplasm.

Autor: de Carvalho LGA; Center for Research and Drug Development (NPDM), Federal University of Ceara, Fortaleza 60020-181, CE, Brazil.; Post-Graduate Program in Translational Medicine, Federal University of Ceara, Fortaleza 60020-181, CE, Brazil., Komoto TT; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-390, SP, Brazil., Moreno DA; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-390, SP, Brazil., Goes JVC; Center for Research and Drug Development (NPDM), Federal University of Ceara, Fortaleza 60020-181, CE, Brazil.; Post-Graduate Program of Pathology, Federal University of Ceara, Fortaleza 60020-181, CE, Brazil., de Oliveira RTG; Center for Research and Drug Development (NPDM), Federal University of Ceara, Fortaleza 60020-181, CE, Brazil.; Post-Graduate Program in Medical Science, Federal University of Ceara, Fortaleza 60020-181, CE, Brazil., de Lima Melo MM; Center for Research and Drug Development (NPDM), Federal University of Ceara, Fortaleza 60020-181, CE, Brazil.; Post-Graduate Program in Medical Science, Federal University of Ceara, Fortaleza 60020-181, CE, Brazil., Roa MEGV; Post-Graduate Program of Immunology, University of São Paulo, São Paulo 14040-902, SP, Brazil., Gonçalves PG; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-390, SP, Brazil.; Department of Pathology, School of Medicine, Universidade Estadual Paulista, Botucatu 18618-970, SP, Brazil., Montefusco-Pereira CV; Center for Research and Drug Development (NPDM), Federal University of Ceara, Fortaleza 60020-181, CE, Brazil., Pinheiro RF; Center for Research and Drug Development (NPDM), Federal University of Ceara, Fortaleza 60020-181, CE, Brazil.; Post-Graduate Program in Translational Medicine, Federal University of Ceara, Fortaleza 60020-181, CE, Brazil.; Post-Graduate Program of Pathology, Federal University of Ceara, Fortaleza 60020-181, CE, Brazil.; Post-Graduate Program in Medical Science, Federal University of Ceara, Fortaleza 60020-181, CE, Brazil., Ribeiro Junior HL; Center for Research and Drug Development (NPDM), Federal University of Ceara, Fortaleza 60020-181, CE, Brazil.; Post-Graduate Program in Translational Medicine, Federal University of Ceara, Fortaleza 60020-181, CE, Brazil.; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-390, SP, Brazil.; Post-Graduate Program in Medical Science, Federal University of Ceara, Fortaleza 60020-181, CE, Brazil.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Jun 13; Vol. 24 (12). Date of Electronic Publication: 2023 Jun 13.
DOI: 10.3390/ijms241210058
Abstrakt: The aim of this study was to evaluate the expression of USP7 , USP15 , UBE2O , and UBE2T genes in Myelodysplastic neoplasm (MDS) to identify possible targets of ubiquitination and deubiquitination in MDS pathobiology. To achieve this, eight datasets from the Gene Expression Omnibus (GEO) database were integrated, and the expression relationship of these genes was analyzed in 1092 MDS patients and healthy controls. Our results showed that UBE2O , UBE2T , and USP7 were upregulated in MDS patients compared with healthy individuals, but only in mononucleated cells collected from bone marrow samples ( p < 0.001). In contrast, only the USP15 gene showed a downregulated expression compared with healthy individuals ( p = 0.03). Additionally, the upregulation of UBE2T expression was identified in MDS patients with chromosomal abnormalities compared with patients with normal karyotypes ( p = 0.0321), and the downregulation of UBE2T expression was associated with MDS hypoplastic patients ( p = 0.033). Finally, the USP7 and USP15 genes were strongly correlated with MDS (r = 0.82; r2 = 0.67; p < 0.0001). These findings suggest that the differential expression of the USP15-USP7 axis and UBE2T may play an important role in controlling genomic instability and the chromosomal abnormalities that are a striking characteristic of MDS.
Databáze: MEDLINE
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