Quantitative Detection of Viable but Nonculturable Vibrio parahaemolyticus in Frozen Bivalve Molluscs.
| Autor: | Di Salvo E; Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, Viale Palatucci snc, 98168 Messina, Italy., Panebianco F; Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, Italy., Panebianco A; Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, Viale Palatucci snc, 98168 Messina, Italy., Ziino G; Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, Viale Palatucci snc, 98168 Messina, Italy. |
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| Jazyk: | angličtina |
| Zdroj: | Foods (Basel, Switzerland) [Foods] 2023 Jun 15; Vol. 12 (12). Date of Electronic Publication: 2023 Jun 15. |
| DOI: | 10.3390/foods12122373 |
| Abstrakt: | Vibrio parahaemolyticus is a foodborne pathogen diffusely distributed in the marine environment and often isolated from raw seafood belonging to different species, mostly shellfish. Ingestion of under- or uncooked seafood contaminated by V. parahaemolyticus can cause severe gastrointestinal symptoms in humans. Due to its ability to withstand low temperatures, Vibrio spp. could survive in frozen seafoods for long periods by entering the viable but nonculturable state (VBNC) and may constitute an unrecognized source of food contamination and infection. In the present study, seventy-seven frozen bivalve molluscs (35 mussels; 42 clams) were subjected to the detection and enumeration of viable V. parahaemolyticus using standard culture methods. VBNC forms were detected and quantified by applying an optimized protocol based on Propidium Monoazide (PMA) and Quantitative PCR (qPCR). All samples were negative for both the detection and enumeration of V. parahaemolyticus by the standard culture methods. VBNC forms were detected in 11.7% of the samples (9/77), with values ranging from 1.67 to 2.29 Log CFU/g. Only clam samples were positive for the detection of VBNC forms. The results of this study highlighted that VBNC V. parahaemolyticus may be present in frozen bivalve molluscs. Further data on the prevalence of VBNC V. parahaemolyticus in frozen seafood are needed in order to perform a robust risk assessment. |
| Databáze: | MEDLINE |
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