Airway Epithelial Cultures of Children with Esophageal Atresia as a Model to Study Respiratory Tract Disorders.

Autor:	Dreyer HHM; Department of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands.; Regenerative Medicine Center Utrecht, University Medical Center, Utrecht University, 3584 CX Utrecht, The Netherlands., van Tuyll van Serooskerken ES; Department of Pediatric Surgery, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands., Rodenburg LW; Department of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands.; Regenerative Medicine Center Utrecht, University Medical Center, Utrecht University, 3584 CX Utrecht, The Netherlands., Bittermann AJN; Pediatric Upper Gastrointestinal and Airway Treatment Center, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands.; Department of Pediatric Otorhinolaryngology, Pediatric Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands., Arets HGM; Department of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands.; Pediatric Upper Gastrointestinal and Airway Treatment Center, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands., Reuling EMBP; Department of Pediatric Surgery, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands.; Pediatric Upper Gastrointestinal and Airway Treatment Center, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands., Verweij JW; Department of Pediatric Surgery, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands.; Pediatric Upper Gastrointestinal and Airway Treatment Center, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands., Haarman EG; Department of Paediatric Pulmonology, Emma Children's Hospital, Amsterdam UMC, 1105 AZ Amsterdam, The Netherlands., van der Zee DC; Department of Pediatric Surgery, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands.; Pediatric Upper Gastrointestinal and Airway Treatment Center, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands., Tytgat SHAJ; Department of Pediatric Surgery, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands.; Pediatric Upper Gastrointestinal and Airway Treatment Center, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands., van der Ent CK; Department of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands., Beekman JM; Department of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands.; Regenerative Medicine Center Utrecht, University Medical Center, Utrecht University, 3584 CX Utrecht, The Netherlands., Amatngalim GD; Department of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands.; Regenerative Medicine Center Utrecht, University Medical Center, Utrecht University, 3584 CX Utrecht, The Netherlands., Lindeboom MYA; Department of Pediatric Surgery, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands.; Pediatric Upper Gastrointestinal and Airway Treatment Center, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands.
Jazyk:	angličtina
Zdroj:	Children (Basel, Switzerland) [Children (Basel)] 2023 Jun 05; Vol. 10 (6). Date of Electronic Publication: 2023 Jun 05.
DOI:	10.3390/children10061020
Abstrakt:	Esophageal atresia (EA) is a rare birth defect in which respiratory tract disorders are a major cause of morbidity. It remains unclear whether respiratory tract disorders are in part caused by alterations in airway epithelial cell functions such as the activity of motile cilia. This can be studied using airway epithelial cell culture models of patients with EA. Therefore, the aim of this study was to evaluate the feasibility to culture and functionally characterize motile cilia function in the differentiated air-liquid interface cultured airway epithelial cells and 3D organoids derived from nasal brushings and bronchoalveolar lavage (BAL) fluid from children with EA. We demonstrate the feasibility of culturing differentiated airway epithelia and organoids of nasal brushings and BAL fluid of children with EA, which display normal motile cilia function. EA patient-derived airway epithelial cultures can be further used to examine whether alterations in epithelial functions contribute to respiratory disorders in EA.
Databáze:	MEDLINE
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