| Autor: |
Sathi FA; Department of Microbiology, Mymensingh Medical College, Mymensingh 2200, Bangladesh., Aung MS; Department of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060-8556, Japan., Paul SK; Netrokona Medical College, Netrokona 2400, Bangladesh., Nasreen SA; Department of Microbiology, Mymensingh Medical College, Mymensingh 2200, Bangladesh., Haque N; Department of Microbiology, Mymensingh Medical College, Mymensingh 2200, Bangladesh., Roy S; Department of Microbiology, Mymensingh Medical College, Mymensingh 2200, Bangladesh., Ahmed S; Mugda Medical College, Dhaka 1214, Bangladesh., Alam MM; Department of ENT, Mymensingh Medical College Hospital, Mymensingh 2200, Bangladesh., Khan S; Department of Oral Microbiology, Mymensingh Medical College Hospital, Mymensingh 2200, Bangladesh., Rabbany MA; Department of Neonatology, Mymensingh Medical College Hospital, Mymensingh 2200, Bangladesh., Biswas JP; Department of Pathology, Netrokona Medical College, Netrokona 2400, Bangladesh., Kobayashi N; Department of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060-8556, Japan. |
| Abstrakt: |
Candida auris , Candida blankii , and Kodamaea ohmeri have been regarded as emerging fungal pathogens that can cause infections with high mortality. For genotyping of C. auris , a multilocus sequence typing (MLST) scheme based on four locus sequences has been reported, while there is no typing scheme for C. blankii and K. ohmeri . In the present study, the existing MLST scheme of C. auris was modified by adding more locus types deduced from sequence data available in the GenBank database. Furthermore, MLST schemes of C. blankii and K. ohmeri were developed using the four cognate loci (ITS, RPB1 , RPB2 , D1/D2) and similar sequence regions to those of C. auris . These MLST schemes were applied to identify the ST (sequence type) of clinical isolates of C. auris ( n = 7), C. blankii ( n = 9), and K. ohmeri ( n = 6), derived from septicemia or otomycosis in Bangladesh in 2021. All the C. auris isolates were classified into a single ST (ST5) and clade I, having a Y132F substitution in ERG11p, which is associated with azole resistance. Similarly, all the C. blankii isolates belonged to a single type (ST1). In contrast, six K. ohmeri isolates were assigned to five types (ST1-ST5), suggesting its higher genetic diversity. These findings revealed the availability of MLST schemes for these three fungal species for understanding their clonal diversity among clinical isolates. |
