Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO 3 Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects.

Autor: Adolpho LF; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, Brazil., Ribeiro LMS; Institute of Physics and Chemistry, University of Itajubá, Itajubá 37500-903, MG, Brazil., Freitas GP; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, Brazil.; School of Dentistry, Federal University of Goiás, Goiânia 74605-020, GO, Brazil., Lopes HB; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, Brazil., Gomes MPO; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, Brazil., Ferraz EP; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, Brazil., Gimenes R; Institute of Physics and Chemistry, University of Itajubá, Itajubá 37500-903, MG, Brazil., Beloti MM; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, Brazil., Rosa AL; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, Brazil.
Jazyk: angličtina
Zdroj: Journal of functional biomaterials [J Funct Biomater] 2023 Jun 01; Vol. 14 (6). Date of Electronic Publication: 2023 Jun 01.
DOI: 10.3390/jfb14060306
Abstrakt: Background: Tissue engineering and cell therapy have been the focus of investigations on how to treat challenging bone defects. This study aimed to produce and characterize a P(VDF-TrFE)/BaTiO 3 scaffold and evaluate the effect of mesenchymal stem cells (MSCs) combined with this scaffold and photobiomodulation (PBM) on bone repair.
Methods and Results: P(VDF-TrFE)/BaTiO 3 was synthesized using an electrospinning technique and presented physical and chemical properties suitable for bone tissue engineering. This scaffold was implanted in rat calvarial defects (unilateral, 5 mm in diameter) and, 2 weeks post-implantation, MSCs were locally injected into these defects ( n = 12/group). Photobiomodulation was then applied immediately, and again 48 and 96 h post-injection. The μCT and histological analyses showed an increment in bone formation, which exhibited a positive correlation with the treatments combined with the scaffold, with MSCs and PBM inducing more bone repair, followed by the scaffold combined with PBM, the scaffold combined with MSCs, and finally the scaffold alone (ANOVA, p ≤ 0.05).
Conclusions: The P(VDF-TrFE)/BaTiO 3 scaffold acted synergistically with MSCs and PBM to induce bone repair in rat calvarial defects. These findings emphasize the need to combine a range of techniques to regenerate large bone defects and provide avenues for further investigations on innovative tissue engineering approaches.
Databáze: MEDLINE
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