NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice.

Autor: Tran LS; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia.; Department of Molecular and Translational Science, Monash University, Melbourne, VIC, Australia., Ying L; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia.; Department of Molecular and Translational Science, Monash University, Melbourne, VIC, Australia., D'Costa K; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia., Wray-McCann G; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia., Kerr G; Department of Anatomy and Developmental Biology, Development and Stem Cells Program, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia., Le L; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia.; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia., Allison CC; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia., Ferrand J; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia., Chaudhry H; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia., Emery J; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia.; Department of Molecular and Translational Science, Monash University, Melbourne, VIC, Australia., De Paoli A; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia., Colon N; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia., Creed S; Monash Micro Imaging, Monash University, Melbourne, VIC, Australia., Kaparakis-Liaskos M; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia., Como J; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia., Dowling JK; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia., Johanesen PA; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia., Kufer TA; Department of Immunology, University of Hohenheim, Institute of Nutritional Medicine, Stuttgart, Germany., Pedersen JS; TissuPath, Melbourne, VIC, Australia., Mansell A; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia.; Department of Molecular and Translational Science, Monash University, Melbourne, VIC, Australia., Philpott DJ; Department of Immunology, University of Toronto, Toronto, ON, Canada., Elgass KD; Monash Micro Imaging, Monash University, Melbourne, VIC, Australia., Abud HE; Department of Anatomy and Developmental Biology, Development and Stem Cells Program, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia., Nachbur U; Cell Signalling and Cell Death Division, WEHI, Melbourne, VIC, Australia., Croker BA; Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.; Inflammation Division, WEHI, Melbourne, VIC, Australia., Masters SL; Inflammation Division, WEHI, Melbourne, VIC, Australia., Ferrero RL; Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Melbourne, VIC, Australia. Richard.Ferrero@Hudson.org.au.; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia. Richard.Ferrero@Hudson.org.au.; Inflammation Division, WEHI, Melbourne, VIC, Australia. Richard.Ferrero@Hudson.org.au.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Jun 26; Vol. 14 (1), pp. 3804. Date of Electronic Publication: 2023 Jun 26.
DOI: 10.1038/s41467-023-39487-1
Abstrakt: The interleukin-1 family members, IL-1β and IL-18, are processed into their biologically active forms by multi-protein complexes, known as inflammasomes. Although the inflammasome pathways that mediate IL-1β processing in myeloid cells have been defined, those involved in IL-18 processing, particularly in non-myeloid cells, are still not well understood. Here we report that the host defence molecule NOD1 regulates IL-18 processing in mouse epithelial cells in response to the mucosal pathogen, Helicobacter pylori. Specifically, NOD1 in epithelial cells mediates IL-18 processing and maturation via interactions with caspase-1, instead of the canonical inflammasome pathway involving RIPK2, NF-κB, NLRP3 and ASC. NOD1 activation and IL-18 then help maintain epithelial homoeostasis to mediate protection against pre-neoplastic changes induced by gastric H. pylori infection in vivo. Our findings thus demonstrate a function for NOD1 in epithelial cell production of bioactive IL-18 and protection against H. pylori-induced pathology.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz: