Basement membrane properties and their recapitulation in organ-on-chip applications.

Autor:	Salimbeigi G; School of Mechanical and Manufacturing Engineering, Dublin City University, Dublin 9, Ireland., Vrana NE; Spartha Medical, 14B Rue de la Canardiere, 67100, Strasbourg, France.; National Institute of Health and Medical Research, INSERM UMR1121, Biomaterials and Bioengineering, 11 Rue Humann, 67000, Strasbourg, France., Ghaemmaghami AM; Immunology & Immuno-bioengineering Group, School of Life Sciences, Faculty of Medicine & Health Sciences, Nottingham, University of Nottingham, NG7 2RD, UK., Huri PY; Ankara University, Faculty of Engineering, Department of Biomedical Engineering, Ankara, 06560, Turkey., McGuinness GB; School of Mechanical and Manufacturing Engineering, Dublin City University, Dublin 9, Ireland.
Jazyk:	angličtina
Zdroj:	Materials today. Bio [Mater Today Bio] 2022 May 23; Vol. 15, pp. 100301. Date of Electronic Publication: 2022 May 23 (Print Publication: 2022).
DOI:	10.1016/j.mtbio.2022.100301
Abstrakt:	Drug discovery and toxicology is a complex process that involves considerable basic research and preclinical evaluation. These depend highly on animal testing which often fails to predict human trial outcomes due to species differences. Coupled with ethical concerns around animal testing, this leads to a high demand for improved in vitro cell culture platforms. Current research efforts, in this regard, however, are facing a challenge to provide physiologically relevant in vitro human organ models for a reliable assessment of the physiological responses of the body to drug compounds and toxins. The latest development in in vitro cell culture models, organ-on-chips (OOCs), seek to introduce more realistic models of organ function. Current OOCs often use commercial porous polymeric membranes as a barrier membrane for cell culture which is challenging due to the poor replication of the physiological architectures. Better recapitulation of the native basement membrane (BM) characteristics is desirable for modelling physical (e.g. intestine, skin and lung) and metabolic (e.g. liver) barrier models. In this review, the relevance of the physical and mechanical properties of the membrane to cell and system behaviour is elucidated. Key parameters for replicating the BM are also described. This review provides information for future development of barrier organ models focusing on BM-mimicking substrates as a core structure. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2022 The Authors.)
Databáze:	MEDLINE
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